Introduction

SP-Designer is an open-source program providing a user-friendly tool for the design of specific PCR primer pairs from a DNA sequence alignment containing sequences from various taxa. SP-Designer selects PCR primer pairs for the amplification of DNA from a target species on the basis of several criteria: (i) primer specificity, as assessed by interspecific sequence polymorphism in the annealing regions, (ii) the biochemical characteristics of the primers and (iii) the intended PCR conditions. SP-Designer generates tables, detailing the primer pair and PCR characteristics, and a FASTA file locating the primer sequences in the original sequence alignment. SP-Designer is Windows-compatible and freely available from http://www2.sophia.inra.fr/urih/sophia_mart/sp_designer/info_sp_designer.php.

Publications

  1. SP-Designer: a user-friendly program for designing species-specific primer pairs from DNA sequence alignments.
    Cite this
    Villard P, Malausa T, 2013-07-01 - Molecular ecology resources

Credits

  1. Pierre Villard
    Developer

    Institut Universitaire de Technologie de Perpignan, Département STID, France

  2. Thibaut Malausa
    Investigator

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Summary
AccessionBT005437
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByThibaut Malausa