Introduction

We propose a joint experimental and theoretical approach to the automated reconstruction of elemental fluxes in microbial communities. While stable isotope probing of proteins (protein-SIP) has been successfully applied to study interactions and elemental carbon and nitrogen fluxes, the volume and complexity of mass spectrometric data in protein-SIP experiments pose new challenges for data analysis. Together with a flexible experimental setup, the novel bioinformatics tool MetaProSIP offers an automated high-throughput solution for a wide range of (13)C or (15)N protein-SIP experiments with special emphasis on the analysis of metaproteomic experiments where differential labeling of organisms can occur. The information calculated in MetaProSIP includes the determination of multiple relative isotopic abundances, the labeling ratio between old and new synthesized proteins, and the shape of the isotopic distribution. These parameters define the metabolic capacities and dynamics within the investigated microbial culture. MetaProSIP features a high degree of reproducibility, reliability, and quality control reporting. The ability to embed into the OpenMS framework allows for flexible construction of custom-tailored workflows. Software and documentation are available under an open-source license at www.openms.de/MetaProSIP.

Publications

  1. MetaProSIP: automated inference of stable isotope incorporation rates in proteins for functional metaproteomics.
    Cite this
    Sachsenberg T, Herbst FA, Taubert M, Kermer R, Jehmlich N, von Bergen M, Seifert J, Kohlbacher O, 2015-02-01 - Journal of proteome research

Credits

  1. Timo Sachsenberg
    Developer

    Applied Bioinformatics, Center for Bioinformatics, Germany

  2. Florian-Alexander Herbst
    Developer

  3. Martin Taubert
    Developer

  4. René Kermer
    Developer

  5. Nico Jehmlich
    Developer

  6. Martin von Bergen
    Developer

  7. Jana Seifert
    Developer

  8. Oliver Kohlbacher
    Investigator

Community Ratings

UsabilityEfficiencyReliabilityRated By
0 user
Sign in to rate
Summary
AccessionBT005568
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByOliver Kohlbacher