Introduction

A software tool, Sweet Substitute, is described, which assists tandem mass spectrometry (MS/MS)-based glycosylation characterization from within a tryptic digest. The algorithm creates a virtual nanoelectrospray-quadrupole time-of-flight style-MS/MS spectrum of any user-defined N-linked glycan structure. An empirical peak height modeling routine is implemented in the program. By comparing the theoretical MS/MS data with the deconvoluted and deisotoped experimental MS/MS data, the user is able to quickly assess whether a proposed candidate oligosaccharide structure is a plausible one.

Publications

  1. Sweet Substitute: a software tool for in silico fragmentation of peptide-linked N-glycans.
    Cite this
    Clerens S, Van den Ende W, Verhaert P, Geenen L, Arckens L, 2004-03-01 - Proteomics

Credits

  1. Stefan Clerens
    Developer

  2. Wim Van den Ende
    Developer

  3. Peter Verhaert
    Developer

  4. Lieve Geenen
    Developer

  5. Lutgarde Arckens
    Investigator

Community Ratings

UsabilityEfficiencyReliabilityRated By
0 user
Sign in to rate
Summary
AccessionBT005731
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByLutgarde Arckens