Introduction

RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.

Publications

  1. Analysis of cell-based RNAi screens.
    Cite this
    Boutros M, Brás LP, Huber W, 2006-01-01 - Genome biology

Credits

  1. Michael Boutros
    Developer

    Signaling and Functional Genomics, German Cancer Research Center

  2. Lígia P Brás
    Developer

  3. Wolfgang Huber
    Investigator

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Summary
AccessionBT006419
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesR
User InterfaceTerminal Command Line
Download Count0
Submitted ByWolfgang Huber