Introduction

Modern microscope automation permits the collection of vast amounts of continuous anatomical imagery in both two and three dimensions. These large data sets present significant challenges for data storage, access, viewing, annotation and analysis. The cost and overhead of collecting and storing the data can be extremely high. Large data sets quickly exceed an individual's capability for timely analysis and present challenges in efficiently applying transforms, if needed. Finally annotated anatomical data sets can represent a significant investment of resources and should be easily accessible to the scientific community. The Viking application was our solution created to view and annotate a 16.5 TB ultrastructural retinal connectome volume and we demonstrate its utility in reconstructing neural networks for a distinctive retinal amacrine cell class. Viking has several key features. (1) It works over the internet using HTTP and supports many concurrent users limited only by hardware. (2) It supports a multi-user, collaborative annotation strategy. (3) It cleanly demarcates viewing and analysis from data collection and hosting. (4) It is capable of applying transformations in real-time. (5) It has an easily extensible user interface, allowing addition of specialized modules without rewriting the viewer.

Publications

  1. The Viking viewer for connectomics: scalable multi-user annotation and summarization of large volume data sets.
    Cite this
    Anderson JR, Mohammed S, Grimm B, Jones BW, Koshevoy P, Tasdizen T, Whitaker R, Marc RE, 2011-01-01 - Journal of microscopy

Credits

  1. J R Anderson
    Developer

    Department of Ophthalmology, Moran Eye Center

  2. S Mohammed
    Developer

  3. B Grimm
    Developer

  4. B W Jones
    Developer

  5. P Koshevoy
    Developer

  6. T Tasdizen
    Developer

  7. R Whitaker
    Developer

  8. R E Marc
    Investigator

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Summary
AccessionBT006478
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByR E Marc