Introduction

Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.

Publications

  1. Frealign: An Exploratory Tool for Single-Particle Cryo-EM.
    Cite this
    Grigorieff N, 2016-01-01 - Methods in enzymology
  2. Likelihood-based classification of cryo-EM images using FREALIGN.
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    Lyumkis D, Brilot AF, Theobald DL, Grigorieff N, 2013-09-01 - Journal of structural biology
  3. GPU-enabled FREALIGN: accelerating single particle 3D reconstruction and refinement in Fourier space on graphics processors.
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    Li X, Grigorieff N, Cheng Y, 2010-12-01 - Journal of structural biology
  4. FREALIGN: high-resolution refinement of single particle structures.
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    Grigorieff N, 2007-01-01 - Journal of structural biology
  5. Noise bias in the refinement of structures derived from single particles.
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    Stewart A, Grigorieff N, 2004-12-01 - Ultramicroscopy

Credits

  1. N Grigorieff
    Investigator

    Janelia Research Campus, Howard Hughes Medical Institute, United States of America

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Summary
AccessionBT006564
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Country/RegionUnited States of America
Submitted ByN Grigorieff