Introduction

L-Measure (LM) is a freely available software tool for the quantitative characterization of neuronal morphology. LM computes a large number of neuroanatomical parameters from 3D digital reconstruction files starting from and combining a set of core metrics. After more than six years of development and use in the neuroscience community, LM enables the execution of commonly adopted analyses as well as of more advanced functions. This report illustrates several LM protocols: (i) extraction of basic morphological parameters, (ii) computation of frequency distributions, (iii) measurements from user-specified subregions of the neuronal arbors, (iv) statistical comparison between two groups of cells and (v) filtered selections and searches from collections of neurons based on any Boolean combination of the available morphometric measures. These functionalities are easily accessed and deployed through a user-friendly graphical interface and typically execute within few minutes on a set of approximately 20 neurons. The tool is available at http://krasnow.gmu.edu/cn3 for either online use on any Java-enabled browser and platform or download for local execution under Windows and Linux.

Publications

  1. L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies.
    Cite this
    Scorcioni R, Polavaram S, Ascoli GA, 2008-01-01 - Nature protocols

Credits

  1. Ruggero Scorcioni
    Developer

    Krasnow Institute for Advanced Study, George Mason University, United States of America

  2. Sridevi Polavaram
    Developer

  3. Giorgio A Ascoli
    Investigator

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Summary
AccessionBT006603
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByGiorgio A Ascoli