Introduction

This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.

Publications

  1. SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.
    Cite this
    Shaikh TR, Gao H, Baxter WT, Asturias FJ, Boisset N, Leith A, Frank J, 2008-01-01 - Nature protocols

Credits

  1. Tanvir R Shaikh
    Developer

    Wadsworth Center, Empire State Plaza, United States of America

  2. Haixiao Gao
    Developer

  3. William T Baxter
    Developer

  4. Francisco J Asturias
    Developer

  5. Nicolas Boisset
    Developer

  6. Ardean Leith
    Developer

  7. Joachim Frank
    Investigator

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Summary
AccessionBT006728
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesPerl
User InterfaceTerminal Command Line
Download Count0
Submitted ByJoachim Frank