Introduction

DNA methylation is an important epigenetic modification involved in gene regulation, which can now be measured using whole-genome bisulfite sequencing. However, cost, complexity of the data, and lack of comprehensive analytical tools are major challenges that keep this technology from becoming widely applied. Here we present BSmooth, an alignment, quality control and analysis pipeline that provides accurate and precise results even with low coverage data, appropriately handling biological replicates. BSmooth is open source software, and can be downloaded from http://rafalab.jhsph.edu/bsmooth.

Publications

  1. BSmooth: from whole genome bisulfite sequencing reads to differentially methylated regions.
    Cite this
    Hansen KD, Langmead B, Irizarry RA, 2012-10-01 - Genome biology

Credits

  1. Kasper D Hansen
    Developer

  2. Benjamin Langmead
    Developer

  3. Rafael A Irizarry
    Investigator

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Summary
AccessionBT006808
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByRafael A Irizarry