Introduction

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

Publications

  1. A map of nucleosome positions in yeast at base-pair resolution.
    Cite this
    Brogaard K, Xi L, Wang JP, Widom J, 2012-06-01 - Nature

Credits

  1. Kristin Brogaard
    Developer

    Department of Molecular Biosciences, Northwestern University, United States of America

  2. Liqun Xi
    Developer

  3. Ji-Ping Wang
    Developer

  4. Jonathan Widom
    Investigator

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Summary
AccessionBT006843
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesR
User InterfaceTerminal Command Line
Download Count0
Submitted ByJonathan Widom