Introduction

The advent of next-generation sequencing (NGS) has created unprecedented opportunities to examine viral populations within individual hosts, among infected individuals and over time. Comparing sequence variability across viral genomes allows for the construction of complex population structures, the analysis of which can yield powerful biological insights. However, the simultaneous display of sequence variation, coverage depth and quality scores across thousands of bases presents a unique visualization challenge that has not been fully met by current NGS analysis tools.Here, we present LayerCake, a self-contained visualization tool that allows for the rapid analysis of variation in viral NGS data. LayerCake enables the user to simultaneously visualize variations in multiple viral populations across entire genomes within a highly customizable framework, drawing attention to pertinent and interesting patterns of variation. We have successfully deployed LayerCake to assist with a variety of different genomics datasets.Program downloads and detailed instructions are available at http://graphics.cs.wisc.edu/WP/layercake under a modified MIT license. LayerCake is a cross-platform tool written in the Processing framework for Java.mcorrell@cs.wisc.edu.

Publications

  1. LayerCake: a tool for the visual comparison of viral deep sequencing data.
    Cite this
    Correll M, Bailey AL, Sarikaya A, O'Connor DH, Gleicher M, 2015-11-01 - Bioinformatics (Oxford, England)

Credits

  1. Michael Correll
    Developer

  2. Adam L Bailey
    Developer

    Department of Pathology and Laboratory Medicine, University of Wisconsin, United States of America

  3. Alper Sarikaya
    Developer

  4. David H O'Connor
    Developer

    Department of Pathology and Laboratory Medicine, University of Wisconsin, United States of America

  5. Michael Gleicher
    Investigator

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Summary
AccessionBT006947
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByMichael Gleicher