Introduction

Data-independent acquisition (DIA) in liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) provides comprehensive untargeted acquisition of molecular data. We provide an open-source software pipeline, which we call MS-DIAL, for DIA-based identification and quantification of small molecules by mass spectral deconvolution. For a reversed-phase LC-MS/MS analysis of nine algal strains, MS-DIAL using an enriched LipidBlast library identified 1,023 lipid compounds, highlighting the chemotaxonomic relationships between the algal strains.

Publications

  1. MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis.
    Cite this
    Tsugawa H, Cajka T, Kind T, Ma Y, Higgins B, Ikeda K, Kanazawa M, VanderGheynst J, Fiehn O, Arita M, 2015-06-01 - Nature methods

Credits

  1. Hiroshi Tsugawa
    Developer

    1] RIKEN Center for Sustainable Resource Science, Yokohama, Japan

  2. Tomas Cajka
    Developer

    Genome Center, University of California Davis, United States of America

  3. Tobias Kind
    Developer

    Genome Center, University of California Davis, United States of America

  4. Yan Ma
    Developer

    Genome Center, University of California Davis, United States of America

  5. Brendan Higgins
    Developer

    Department of Biological and Agricultural Engineering, University of California Davis, United States of America

  6. Kazutaka Ikeda
    Developer

    1] RIKEN Center for Integrative Medical Sciences, Yokohama, Japan

  7. Mitsuhiro Kanazawa
    Developer

    Reifycs Inc., Minato-ku, Japan

  8. Jean VanderGheynst
    Developer

    Department of Biological and Agricultural Engineering, University of California Davis, United States of America

  9. Oliver Fiehn
    Developer

    1] Genome Center, University of California Davis, Saudi Arabia

  10. Masanori Arita
    Investigator

    1] RIKEN Center for Sustainable Resource Science, Yokohama, Japan

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Summary
AccessionBT006968
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Country/RegionJapan
Submitted ByMasanori Arita