Applied Bioinformatics Course

Primary structure

• Use EMBOSS Pepstat to calculate amino acid composition of Pisum sativum post-floral specific protein 1 PPF1_PEA
• Use EMBOSS Pepinfor to create flowcharts of Pisum sativum post-floral specific protein 1 PPF1_PEA
• Use ExPASy Protscale to create flowcharts to display various properties of the Pisum sativum post-floral specific protein 1 PPF1_PEA

Secondary structure

• Predict the secondary structure of Human hemoglobin alpha chain HBA_HUMAN and beta chain HBB_HUMAN
• Predict the secondary structure of the N-terminal domain of mouse carcinoembryonic antigen CEAM1_MOUSE
• Predict the potential coiled-coil region of the yeast amino acid biosynthesis general control protein GCN4_YEAST

Transmembrane helics

• Use EMBOSS TMAP and ExPASy TMHMM, TMPred, TopPred, SOUSI to predict the transmembrane helices of the Pisum sativum post-floral specific protein PPF1_PEA
• Use EMBOSS TMAP and ExPASy TMHMM, TMPred, TopPred, SOUSI to predict the transmembrane helices of sheep ovine opsin OPSD_SHEEP
• Use EMBOSS TMAP and ExPASy TMHMM, TMPred, TopPred, SOUSI to predict the transmembrane helices of GCR2 protein NP_175700

Helical wheel

• Draw the four helical wheels for the transmembrane helices of the Pisum sativum post-floral specific protein 1 PPF1_PEA
• Draw the helical wheel for the last two helices of human alpha hemoglobin HBA_HUMAN