Applied Bioinformatics Course

UniProt query

• Find protein sequence of human, mouse and rat hemoglobin alpha chain with entry name: HBA_HUMAN, HBA_MOUSE, HBA_RAT.
• Find 9 human hemoglobin subunits with Advanced Search.
• Find 12 human globins using the Protein family field in Advanced Search.
• Find all entries of alph globin with complete sequence, and find the entries that have three-dimenssional structure.
• Find the pea inner membrane protein PPF-1. Compare the annotations of this entry with HBA_HUAMN.
• Find the Arabidopsis homologs of PPF-1 which belongs to the same family.
• Find all experimentally verified Arabidopsis membrane proteins with potential signal peptide.
• Find the human tumor necrosis factor receptor superfamily member 10b (TRAIL-R2, Death receptor 5).
• Find the TRAIL-R2 (DR5) from macaca fascilacularis (crab-eating macaque, cynomolgus onkey).

Explore the annotation

• Retrieve the mRNA and coding sequence of HBA_HUMAN through cross link to RefSeq.
• Find the literatures related to structure of HBA_HUMAN.
• Make a summary of the general annotations of HBA_HUMAN.
• Find out the alpha helices of HBA_HUMAN.
• Find out the two Histidines of HBA_HUMAN which bind to the heme.
• Make sequence alignment of the 9 human hemoglobin subunits.
• Browse the annotations of the Arabidopsis PPF-1 homolog ALB3_ARATH and compare with that of PPF1_PEA.
• Browse the annotation of human Death Receptor 5 (DR5).
• Retrive coding sequence of crab-eating monkey DR5.