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This tool can perform functional annotation on the mutation site, display the change of the gene, code and amino acid in the mutation site, and the degree of influence on the gene function. In the current version, only ON563414.3 or NC_003310.1 is supported as reference for the online annotation. Both formats of bed and vcf files are supported.
| #CHROM | POS | ID | REF | ALT | QUAL | FILTER | INFO | FORMAT | ON609725.2 |
|---|---|---|---|---|---|---|---|---|---|
| ON563414.3 | 55150 | . | G | A | . | . | . | GT | 1 |
| ON563414.3 | 64443 | . | C | T | . | . | . | GT | 1 |
| ON563414.3 | 133119 | . | TTT | - | . | . | . | GT | 1 |
| ON563414.3 | 167510 | . | G | A | . | . | . | GT | 1 |
| ON563414.3 | 173312 | . | TA | - | . | . | . | GT | 1 |
| ON563414.3 | 190639 | . | G | A | . | . | . | GT | 1 |
| ON563414.3 | 192381 | . | A | AA | . | . | . | GT | 1 |
*Input file(s) should be separated by ‘tab’. The first column is the Chromosome of the reference genome - ON563414.3 or NC_003310.1; the second is the position of the reference genome; the third column is ID; the four column is reference allele; respectively, the fifth column is the base mutation of the virus strain studied by users at the location.