Basic mutant information
Mutant name	gbf1
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	T-DNA insertion_knock out
(Semi-)Dominant/Recessive	recessive
Description	-DNA insertion lines in GBF1 exon 9 (SALK_144534) and intron 4 (SALK_147518) were obtained from the Nottingham Arabidopsis Stock Centre. Homozygous lines were characterized by PCR using gene-specific and T-DNA left border primers (LBb1, 5′-GCGTGGACCGCTTGCTGCAACT-3′; gbf-ex-LP, 5′-GCTTTTGCAGCTTCTACAGTCTTG-3′; gbf-ex-RP, 5′-GGACAGATGCTTGCTGACGGT-3′; gbf-int-LP, 5′-GCACCGAACCTTGGATTTCAC-3′; gbf-int-RP, 5′-TTCCCATCCCCAGTTGGATCT-3′).
References	1: Smykowski A, Zimmermann P, Zentgraf U G-Box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis. Plant Physiol. 2010 Jul;153(3):1321-31
Mutated genes
Locus name	AT4G36730
Alias	GBF1
Organism	Arabidopsis thaliana
Description	G-Box binding factor1
Phenotype information
Natural senescence Leaf color green Marker gene expression no