| Title | Metabolic Recoding of 5'-Methylthioadenosine- Mediated H3K79me1 Demethylation Antagonizes Cadmium-Induced Breast Cancer Progression by Maintaining Autophagic Flux-3 |
|---|---|
| Description | The samples were subsequently loaded onto a 12% separation gel and subjected to SDS-PAGE electrophoresis for protein separation. The target band was excised into 1 mm³ gel pieces, which were then digested with trypsin. An extraction solution was prepared consisting of 5% trifluoroacetic acid (TFA), 50% acetonitrile (ACN), and 45% water to extract the peptides. Desalting was performed using a self-packed desalting column, followed by solvent evaporation in a vacuum centrifugal concentrator at 45°C to prepare for subsequent mass spectrometry analysis. Finally, the peptides were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an Acclaim PepMap RPLC C18 column. The mobile phase consisted of water containing 0.1% formic acid and an 80% ACN solution, with a flow rate of 300 nL/min and an analysis duration of 120 minutes. |
| Organism | Homo sapiens |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA033478 |
| Release Date | 2026-01-03 |
| Submitter | li li (laoweishuju@163.com) |
| Organization | Third Military Medical University(Army Medical University) |
| Submission Date | 2024-12-10 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download |
|---|---|---|---|---|---|---|
| OMIX008223-01 | raw data | 13 | Proteomic Data by Mass Spectrometry (MS) | 5.25 GB | zip |
| Paper Title | Journal Name | Publish Time | Accession | Citing Type |
|---|---|---|---|---|
| 5’‐Methylthioadenosine Metabolic Reprogramming Drives H3K79 Monomethylation‐Mediated PAK2 Upregulation to Promote Cadmium‐Induced Breast Cancer Progression by Impairing Autophagic Flux | Advanced Science | 2025-09 | OMIX008223 OMIX008219 | Deposit |