| Title |
Processed data of H3K9me2 Cut&Tag analysis for E11.5-E15.5 female germ cells |
| Description |
Paired-end reads were trimmed using Trim Galore (v0.6.10) with default parameters. The trimmed reads were aligned to the mouse reference genome (GRCm39) using Bowtie2 (v2.5.3) with the following parameters: --end-to-end, --no-mixed, --no-discordant. SAM files were converted to BAM files, and unmapped reads as well as reads with an alignment quality score below 10 were filtered out. PCR duplicates were removed using Picard MarkDuplicates (v3.1.1). For peak calling, MACS2 (v2.2.9.1) was used with the parameters --broad and -p 0.01. Differential peak analysis was performed using the DiffBind package (v3.8.4) with a significance threshold of fold-change > 2 and P-value < 0.05. Peaks were annotated using the ChIPseeker package (v1.34.1) in combination with the mouse genome annotation database. The similarity between groups was then evaluated using multiBigwigSummary followed by plotCorrelation from deepTools (v3.5.5) |
| Organism |
Mus musculus |
| Data Type |
Genome Binding/Occupancy Profiling |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA033553 |
| Release Date |
2025-06-19 |
| Submitter |
Yi Na Zhang (zhangyina22@mails.ucas.ac.cn) |
| Organization |
Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology |
| Submission Date |
2024-12-31 |