OMIX010702

1Summary

Title	Gut microbiota induces dysspermatogenesis via microbial-derived phenylacetylglycine
Description	This study demonstrates that male gamma-glutamyl transferase 1-deletion (Ggt1-/-) mice exhibits infertility phenotypes, including reduced germ and testicular Leydig cell numbers, increased rates of abnormal sperms, and altered reproductive hormone levels. Metabolomic analysis reveals elevated levels of the gut microbial-derived metabolite phenylacetylglycine (PAGly) in serum and testes of Ggt1-/- mice, with in vivo injection experiments confirming its role in impairing spermatogenesis. Fecal metagenomic and metabolomic analyses show that gut microbiota in Ggt1-/- mice induces elevation of phenylacetic acid (PAA), a precursor metabolite of PAGly.
Organism	Mus musculus
Data Type	Metabolome Data by Mass Spectrometry (MS)
Data Accessibility	Open-access
BioProject	PRJCA042012
Release Date	2025-07-18
Submitter	Fenge Li (lifener@mail.hzau.edu.cn)
Organization	Huazhong Agricultural University
Submission Date	2025-06-21

2Files & Download

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File ID	File Title	Number/Samples	File Type	File Size	File Suffix	Download
OMIX010702-01	FECAL_KO_1	1	Metabolome Data by Mass Spectrometry (MS)	122.21 MB	zip	FTP HTTPs
OMIX010702-02	FECAL_KO_2	1	Metabolome Data by Mass Spectrometry (MS)	122.82 MB	zip	FTP HTTPs
OMIX010702-03	FECAL_KO_3	1	Metabolome Data by Mass Spectrometry (MS)	119.24 MB	zip	FTP HTTPs
OMIX010702-04	FECAL_KO_4	1	Metabolome Data by Mass Spectrometry (MS)	121.92 MB	zip	FTP HTTPs
OMIX010702-05	FECAL_KO_5	1	Metabolome Data by Mass Spectrometry (MS)	125 MB	zip	FTP HTTPs
OMIX010702-06	FECAL_KO_6	1	Metabolome Data by Mass Spectrometry (MS)	120.22 MB	zip	FTP HTTPs
OMIX010702-07	FECAL_WT_1	1	Metabolome Data by Mass Spectrometry (MS)	119.33 MB	zip	FTP HTTPs
OMIX010702-08	FECAL_WT_2	1	Metabolome Data by Mass Spectrometry (MS)	116.06 MB	zip	FTP HTTPs
OMIX010702-09	FECAL_WT_3	1	Metabolome Data by Mass Spectrometry (MS)	114.42 MB	zip	FTP HTTPs
OMIX010702-10	FECAL_WT_4	1	Metabolome Data by Mass Spectrometry (MS)	126.11 MB	zip	FTP HTTPs
OMIX010702-11	FECAL_WT_5	1	Metabolome Data by Mass Spectrometry (MS)	121.52 MB	zip	FTP HTTPs
OMIX010702-12	FECAL_WT_6	1	Metabolome Data by Mass Spectrometry (MS)	116.75 MB	zip	FTP HTTPs
OMIX010702-13	TESTIS_KO1	1	Metabolome Data by Mass Spectrometry (MS)	39.22 MB	zip	FTP HTTPs
OMIX010702-14	TESTIS_KO2	1	Metabolome Data by Mass Spectrometry (MS)	40.85 MB	zip	FTP HTTPs
OMIX010702-15	TESTIS_KO3	1	Metabolome Data by Mass Spectrometry (MS)	36.01 MB	zip	FTP HTTPs
OMIX010702-16	TESTIS_KO4	1	Metabolome Data by Mass Spectrometry (MS)	38.87 MB	zip	FTP HTTPs
OMIX010702-17	TESTIS_KO5	1	Metabolome Data by Mass Spectrometry (MS)	40.02 MB	zip	FTP HTTPs
OMIX010702-18	TESTIS_KO6	1	Metabolome Data by Mass Spectrometry (MS)	41.31 MB	zip	FTP HTTPs
OMIX010702-19	TESTIS_QC01	1	Metabolome Data by Mass Spectrometry (MS)	38.73 MB	zip	FTP HTTPs
OMIX010702-20	TESTIS_QC02	1	Metabolome Data by Mass Spectrometry (MS)	39.41 MB	zip	FTP HTTPs
OMIX010702-21	TESTIS_QC03	1	Metabolome Data by Mass Spectrometry (MS)	39.55 MB	zip	FTP HTTPs
OMIX010702-22	TESTIS_WT1	1	Metabolome Data by Mass Spectrometry (MS)	42.55 MB	zip	FTP HTTPs
OMIX010702-23	TESTIS_WT2	1	Metabolome Data by Mass Spectrometry (MS)	42.5 MB	zip	FTP HTTPs
OMIX010702-24	TESTIS_WT3	1	Metabolome Data by Mass Spectrometry (MS)	41.99 MB	zip	FTP HTTPs
OMIX010702-25	TESTIS_WT4	1	Metabolome Data by Mass Spectrometry (MS)	42.18 MB	zip	FTP HTTPs
OMIX010702-26	TESTIS_WT5	1	Metabolome Data by Mass Spectrometry (MS)	42.89 MB	zip	FTP HTTPs
OMIX010702-27	TESTIS_WT6	1	Metabolome Data by Mass Spectrometry (MS)	44.59 MB	zip	FTP HTTPs

3Relevant Publications

Paper Title	Journal Name	Publish Time	Accession	Citing Type
Gut microbiota induces dysspermatogenesis via microbial-derived phenylacetylglycine in Ggt1-deficient mice	Microbiome	2026-04	OMIX010702 OMIX007804 OMIX1000	Deposit Secondary use

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