AEF1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: AEF1
Synonym: ATPF EDITING FACTOR 1; MPR25
Description: Encoding a protein implicated in RNA editing of plastid atpF and mitochondrial nad5, and splicing of atpF in Arabidopsis.
Protein Family: PPR
Subclass: E
Physical Interaction: NA
Construct Structure: PLS-E
Gene ID & Species: AT3G22150 (Arabidopsis thaliana)
Edited Gene(s): atpF    nad5
Editing Type(s): C-to-U (13)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] The dual-targeted RNA editing factor AEF1 is universally conserved among angiosperms and reveals only minor adaptations upon loss of its chloroplast or its mitochondrial target., Plant molecular biology, 2020. [PMID=31797248]
[3] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[4] AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice., The Plant journal : for cell and molecular biology, 2015. [PMID=25585673]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G22150 Plastid
Chloroplast
atpF 92 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_041683aef1-1T-DNA InsertionT-DNA is inserted at nucleotide 252 from the start codon, within the first P motifHomozygousVariegation, retarded growth and pale leavesAerial tissue14 daysPoisoned Primer Extension (PPE) Assay0.00%UneditedAbsent25585673
SALK_041683complemented plants (AEF1)ComplementationNAHomozygousNAAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteRestored25585673
Col-0WTControlControlControlNormalAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteNone25585673
NANANANANANANANANANANANA31797248
Col-0WTControlControlControlNANANARNA-seq91.23%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract10.00%PoorDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract4.00%PoorDecreased28761003
Arabidopsis thaliana AT3G22150 Mitochondrion nad5 1580 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_041683aef1-1T-DNA InsertionT-DNA is inserted at nucleotide 252 from the start codon, within the first P motifHomozygousVariegation, retarded growth and pale leavesAerial tissue14 daysPoisoned Primer Extension (PPE) Assay58.00%MediumDecreased25585673
SALK_041683complemented plants (AEF1)ComplementationNAHomozygousNAAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteRestored25585673
Col-0WTControlControlControlNormalAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteNone25585673
NANANANANANANANANANANANA31797248
Col-0WTControlControlControlNANANARNA-seq93.88%HighNone32098170
Last update: Feb 2026 (version 2.0)