AEF1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: AEF1
Synonym: ATPF EDITING FACTOR 1, MPR25
Description: Encodes a protein implicated in RNA editing of plastid atpF and mitochondrial nad5, and splicing of atpF in Arabidopsis.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT3G22150 (Arabidopsis thaliana)
Edited Gene(s): atpF    nad5
Editing Type(s): C-to-U (13)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] The dual-targeted RNA editing factor AEF1 is universally conserved among angiosperms and reveals only minor adaptations upon loss of its chloroplast or its mitochondrial target, Plant Mol Biol, 2019. [PMID=31797248]
[3] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[4] AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice, The Plant Journal, 2015. [PMID=25585673]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G22150 Plastid
Chloroplast
atpF 92 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_041683aef1-1T-DNA InsertionT-DNA is inserted at nucleotide 252 from the start codon, within the first P motifHomozygousVariegation, retarded growth and pale leavesAerial tissue14 daysPoisoned Primer Extension (PPE) Assay0.00%UneditedAbsent25585673
SALK_041683complemented plants (AEF1)ComplementationNAHomozygousNAAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteRestored25585673
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteNone25585673
NANANANANANANANANANANANA31797248
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq91.23%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract10.00%PoorDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract4.00%PoorDecreased28761003
Arabidopsis thaliana AT3G22150 Mitochondrion nad5 1580 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_041683aef1-1T-DNA InsertionT-DNA is inserted at nucleotide 252 from the start codon, within the first P motifHomozygousVariegation, retarded growth and pale leavesAerial tissue14 daysPoisoned Primer Extension (PPE) Assay58.00%MediumDecreased25585673
SALK_041683complemented plants (AEF1)ComplementationNAHomozygousNAAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteRestored25585673
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentAerial tissue14 daysPoisoned Primer Extension (PPE) Assay100.00%CompleteNone25585673
NANANANANANANANANANANANA31797248
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq93.88%HighNone32098170
Last update: Jul 2021 (version 1.0)