Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Dongjin | WT | Control | Control | No mutant | Normal | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 87.01% | High | None | 32928899 |
| PFG_4A-50341 | osatp4 | A T-DNA insertion | Thermal asymmetric interlaced PCR of the sequences flanking the T-DNA insertion site showed that the T-DNA inserted itself in the coding region, 1,294 bp downstream of the start codon of rice gene Os0 | Homozygous | Seedling blades exhibit an albino phenotype when grown in the cold. The mutants had 13 tillers on average, which was less than that of the wild type (16 tillers). In addition, the mutant exhibited decreases in plant height, panicle length, average grain weight, and yield per plant. The mutant also exhibited reduced leaf width and chlorophyll under natural field conditions. Similarly, a decrease in chlorophyll content was observed in osatp4 when grown at 30°C, however, Fv/Fm, a measure of maximum PSII quantum yield, was unaffected. When grown at 20°C, which confers cold stress on rice (Hasanuzzaman et al., 2018), the chlorophyll content and the Fv/Fm value significantly decreased in osatp4. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | COM1 | Complementation | For transgene complementation, a 5,617-bp wild-type genomic DNA fragment containing the entire OsATP4 coding region with a 3,156-bp upstream region and a 430-bp downstream sequence was amplified using | Homozygous | Mutant plants expressing a transgene encoding OsATP4 were restored to the wild-type phenotype. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 77.01% | High | Restored | 32928899 |
| NA | COM2 | Complementation | For transgene complementation, a 5,617-bp wild-type genomic DNA fragment containing the entire OsATP4 coding region with a 3,156-bp upstream region and a 430-bp downstream sequence was amplified using | Homozygous | Mutant plants expressing a transgene encoding OsATP4 were restored to the wild-type phenotype. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 87.95% | High | Restored | 32928899 |
| NA | COM3 | Complementation | For transgene complementation, a 5,617-bp wild-type genomic DNA fragment containing the entire OsATP4 coding region with a 3,156-bp upstream region and a 430-bp downstream sequence was amplified using | Homozygous | Mutant plants expressing a transgene encoding OsATP4 were restored to the wild-type phenotype. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 93.67% | High | Restored | 32928899 |
| NA | cr-osatp4-1 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | cr-osatp4-2 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | cr-osatp4-3 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | cr-osatp4-4 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | cr-osatp4-5 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
| NA | cr-osatp4-6 | CRISPR-induced mutant | To generate CRISPR-mediated alterations in the SMR domain of OsATP4, the targeting sequences were designed. The target-site sequences were cloned into the single guide RNA expression cassette pYLgRNA | Knockout | Tillers in cr-osatp4 are less than that of the wild type. The cr-osatp4 exhibits decreases in plant height, panicle length. | Seedling | NA | The amplified PCR products were directly sequenced by Sangon | 0.00% | Unedited | Absent | 32928899 |
