CLB19 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Aethionema cordifolium

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Aethionema cordifolium

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Aethionema grandiflorum

JF794509

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Aethionema grandiflorum

JF794509

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Arabidopsis thaliana

AT1G05750

Plastid

clpP

null

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	89.33%	High	None	25649437
SALK_104250	CLB19wt	Complemented	Plants transformed with wild-type CLB19 driven by the same promoter as used to drive expression of the variants.	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	89.80%	High	Restored	25649437
SALK_104250	S2[TD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	89.00%	High	Similar	25649437
SALK_104250	S2[TN]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	87.17%	High	Similar	25649437
SALK_104250	S2[SD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	51.67%	Medium	Decreased	25649437
SALK_104250	S3[TT]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	40.17%	Medium	Decreased	25649437
SALK_104250	S3[TD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	20.67%	Low	Decreased	25649437
SALK_104250	c19	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	0.00%	Unedited	Absent	25649437

Arabidopsis thaliana

AT1G05750

Chloroplast
Plastid

clpP

559

CDS

C-to-U

CAU=>UAU
CAC=>UAC
NA=>NA

H=>Y
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	clb19-1	T-DNA Insertion	T-DNA is inserted at codon 230	NA	Pale-yellow cotyledons and seedling leaves when grown under standard light conditions and dies shortly afterwards	Seedling	NA	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	18657233
SALK_123752	clb19-3	T-DNA Insertion	NA	NA	Seedlings with a pale-yellow phenotype	Seedling	NA	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	18657233
Col-0	Col-0	No treatment	No treatment	No treatment	No treatment	Seedling	NA	Poisoned Primer Extension (PPE) Assay	NA	High	None	18657233
NA	TC	Complementation	NA	NA	NA	Seedling	NA	Poisoned Primer Extension (PPE) Assay	NA	High	Restored	18657233
NA	CLB19-His	Overexpression	NA	NA	NA	NA	NA	Gel electrophoretic mobility shift assays	NA	NA	NA	25980341
NA	CLB19-_E-His	Overexpression	NA	NA	NA	NA	NA	Gel electrophoretic mobility shift assays	NA	NA	NA	25980341
Col-0	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633
NA	NA	No treatment	No treatment	No treatment	NA	NA	NA	NA	NA	NA	None	29879897
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	71.85%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	67.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	7.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	1.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT1G05750

Plastid

rpoA

null

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	75.67%	High	None	25649437
SALK_104250	CLB19wt	Complemented	Plants transformed with wild-type CLB19 driven by the same promoter as used to drive expression of the variants.	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	78.80%	High	Restored	25649437
SALK_104250	S2[TD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	69.17%	High	Similar	25649437
SALK_104250	S2[TN]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	72.67%	High	Similar	25649437
SALK_104250	S2[SD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	49.80%	Medium	Decreased	25649437
SALK_104250	S3[TT]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	73.17%	High	Similar	25649437
SALK_104250	S3[TD]	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	78.00%	High	Similar	25649437
SALK_104250	c19	A T-DNA insertion	A T-DNA insertion (SALK_104250) in At1g05750	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	0.33%	Poor	Decreased	25649437

Arabidopsis thaliana

AT1G05750

Chloroplast
Plastid

rpoA

200

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	clb19-1	T-DNA Insertion	T-DNA is inserted at codon 230	NA	Pale-yellow cotyledons and seedling leaves when grown under standard light conditions and dies shortly afterwards	Seedling	NA	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	18657233
SALK_123752	clb19-3	T-DNA Insertion	NA	NA	Seedlings with a pale-yellow phenotype	Seedling	NA	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	18657233
Col-0	Col-0	No treatment	No treatment	No treatment	No treatment	Seedling	NA	Poisoned Primer Extension (PPE) Assay	NA	High	None	18657233
NA	TC	Complementation	NA	NA	NA	Seedling	NA	Poisoned Primer Extension (PPE) Assay	NA	High	Restored	18657233
NA	CLB19-His	Overexpression	NA	NA	NA	NA	NA	Gel electrophoretic mobility shift assays	NA	NA	NA	25980341
NA	CLB19-_E-His	Overexpression	NA	NA	NA	NA	NA	Gel electrophoretic mobility shift assays	NA	NA	NA	25980341
Col-0	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	50.00%	Medium	NA	22583633
NA	NA	No treatment	No treatment	No treatment	NA	NA	NA	NA	NA	NA	None	29879897
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	75.94%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	74.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	4.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	5.00%	Poor	Decreased	28761003

Arabis hirsuta

JF794510

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02300	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Arabis hirsuta

JF794510

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02300	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	60.00%	High	NA	22583633

Barbarea verna

JF794511

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Barbarea verna

JF794511

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Brassica oleracea

JF794512

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Brassica oleracea

JF794512

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Brassica rapa

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Brassica rapa

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	Complete	NA	22583633

Capsella bursa-pastoris

JF794513

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial # 0051714	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Capsella bursa-pastoris

JF794513

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial # 0051714	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Crucihimalaya wallichii

JF794514

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00500	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Crucihimalaya wallichii

JF794514

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00500	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Draba nemorosa

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02100	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Draba nemorosa

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02100	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	40.00%	Medium	NA	22583633

Hesperis matronalis

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 586611	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Hesperis matronalis

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 586611	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	60.00%	High	NA	22583633

Iberis amara

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 633243	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Iberis amara

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 633243	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Isatis tinctoria

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Isatis tinctoria

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	50.00%	Medium	NA	22583633

Lepidium sativum

JF794515

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Lepidium sativum

JF794515

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Lepidium virginicum

JF794516

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02600	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Lepidium virginicum

JF794516

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02600	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Lobularia maritima

JF794517

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Lobularia maritima

JF794517

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	50.00%	Medium	NA	22583633

Matthiola incana

JF794518

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Matthiola incana

JF794518

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Nasturtium officinale

JF794519

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial #0200327	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Nasturtium officinale

JF794519

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial #0200327	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	70.00%	High	NA	22583633

Olimarabidopsis pumila

JF794520

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00200	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Olimarabidopsis pumila

JF794520

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00200	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Raphanus sativus

JF794521

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Raphanus sativus

JF794521

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Thlaspi arvense

Chloroplast

clpP

559

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ames 29531	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Thlaspi arvense

Chloroplast

rpoA

200

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ames 29531	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Editing Factor:	CLB19
Synonym:	CHLOROPLAST BIOGENESIS 19, PDE247, PIGMENT DEFECTIVE 247
Description:	Encodes a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts.
Protein Family:	PPR
Subclass:	E+
Construct Structure:	PLS-E-E+
Gene ID & Species:	NA (Aethionema cordifolium) JF794509 (Aethionema grandiflorum) AT1G05750 (Arabidopsis thaliana) JF794510 (Arabis hirsuta) JF794511 (Barbarea verna) JF794512 (Brassica oleracea) NA (Brassica rapa) pastoris (Capsella bursa) JF794514 (Crucihimalaya wallichii) NA (Draba nemorosa) NA (Hesperis matronalis) NA (Iberis amara) NA (Isatis tinctoria) JF794515 (Lepidium sativum) JF794516 (Lepidium virginicum) JF794517 (Lobularia maritima) JF794518 (Matthiola incana) JF794519 (Nasturtium officinale) JF794520 (Olimarabidopsis pumila) JF794521 (Raphanus sativus) NA (Thlaspi arvense)
Edited Gene(s):	rpoA clpP
Editing Type(s):	C-to-U (80)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Expected and unexpected evolution of plant RNA editing factors CLB19, CRR28 and RARE1: retention of CLB19 despite a phylogenetically deep loss of its two known editing targets in Poaceae, BMC Evol Biol, 2018. [PMID=29879897] [3] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [4] Functional analysis of the Arabidopsis thaliana CHLOROPLAST BIOGENESIS 19 pentatricopeptide repeat editing protein, New Phytologist, 2015. [PMID=25980341] [5] Predictable alteration of sequence recognition by RNA editing factors from Arabidopsis, Plant Cell, 2015. [PMID=25649437] [6] Molecular evolution of pentatricopeptide repeat genes reveals truncation in species lacking an editing target and structural domains under distinct selective pressures, BMC Evol Biol, 2012. [PMID=22583633] [7] CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts, The Plant Journal, 2008. [PMID=18657233]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details