CREF3 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: CREF3
Synonym: CHLOROPLAST RNA EDITING FACTOR 3
Description: Mediating RNA editing in psbE (Genomic position: 64109)
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: NA
Gene ID & Species: AT3G14330 (Arabidopsis thaliana)
Edited Gene(s): psbE
Editing Type(s): C-to-U (5)
Publication(s): [1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114]
[2] Pentatricopeptide repeat proteins involved in plant organellar RNA editing, RNA Biol, 2013. [doi:10.4161/rna.24908]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G14330 Chloroplast psbE 214 CDS NA NA NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlNo mutantNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract79.00%HighDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract89.00%HighSimilar28761003
Arabidopsis thaliana AT3G14330 Chloroplast psbE NA NA NA NA NA 64109 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColombiaWTControlControlNo mutantNormalLeaf4 weeksRT-PCR and direct sequencing100.00%CompleteNone23669716
Salk_077977Salk_077977T-DNA insertionAn insertion of the T-DNA in the coding regions of the transgenic lineNAThe T-DNA line displayed no aberrant macroscopic phenotype including germination, growth, leaf color and seed setting ratio under our growth conditionsLeaf4 weeksRT-PCR and direct sequencing0.00%UneditedAbsent23669716
Last update: Sep 2026 (version 2.0)