CREF3 - Plant Editosome Database

Editing Factor:	CREF3
Synonym:	CHLOROPLAST RNA EDITING FACTOR 3
Description:	Encoding a pentatricopeptide repeat protein involved in chloroplast mRNA editing; Mutants display defects in C-U editing of psbE
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	NA
Gene ID & Species:	AT3G14330 (Arabidopsis thaliana)
Edited Gene(s):	psbE
Editing Type(s):	C-to-U (6)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Pentatricopeptide repeat proteins involved in plant organellar RNA editing., RNA biology, 2013. [PMID=23669716]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G14330

Plastid
Chloroplast

214

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.53%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	93.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	79.00%	High	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	89.00%	High	Similar	28761003

Arabidopsis thaliana

AT3G14330

Chloroplast

64109

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Colombia	WT	Control	Control	Control	Normal	Leaf	4 weeks	RT-PCR and direct sequencing	100.00%	Complete	None	23669716
Salk_077977	Salk_077977	T-DNA insertion	An insertion of the T-DNA in the coding regions of the transgenic line	NA	The T-DNA line displayed no aberrant macroscopic phenotype including germination, growth, leaf color and seed setting ratio under our growth conditions	Leaf	4 weeks	RT-PCR and direct sequencing	0.00%	Unedited	Absent	23669716