CREF3 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: CREF3
Synonym: CHLOROPLAST RNA EDITING FACTOR 3
Description: Encoding a pentatricopeptide repeat protein involved in chloroplast mRNA editing; Mutants display defects in C-U editing of psbE
Protein Family: PPR
Subclass: DYW
Construct Structure: NA
Gene ID & Species: AT3G14330 (Arabidopsis thaliana)
Edited Gene(s): psbE
Editing Type(s): C-to-U (6)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Pentatricopeptide repeat proteins involved in plant organellar RNA editing., RNA biology, 2013. [PMID=23669716]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G14330 Plastid
Chloroplast
psbE 214 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq99.53%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract79.00%HighDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract89.00%HighSimilar28761003
Arabidopsis thaliana AT3G14330 Chloroplast psbE NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColombiaWTControlControlControlNormalLeaf4 weeksRT-PCR and direct sequencing100.00%CompleteNone23669716
Salk_077977Salk_077977T-DNA insertionAn insertion of the T-DNA in the coding regions of the transgenic lineNAThe T-DNA line displayed no aberrant macroscopic phenotype including germination, growth, leaf color and seed setting ratio under our growth conditionsLeaf4 weeksRT-PCR and direct sequencing0.00%UneditedAbsent23669716
Last update: Jul 2021 (version 1.0)