CRR21 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: CRR21
Synonym: CHLORORESPIRATORY REDUCTION 21
Description: Encoding a member of the E+ subgroup of the PPR protein family, containing the E and E+ motifs following a tandem array of PPR motifs; CRR21 is required for editing ndhD in the chloroplast and the PG box of CRR21 is essential for editing
Protein Family: PPR
Subclass: E+
Physical Interaction: NA
Construct Structure: PLS-E-E+
Gene ID & Species: AT5G55740 (Arabidopsis thaliana)
Edited Gene(s): ndhD
Editing Type(s): C-to-U (8)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Identification of two pentatricopeptide repeat genes required for RNA editing and zinc binding by C-terminal cytidine deaminase-like domains., The Journal of biological chemistry, 2013. [PMID=24194514]
[3] Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing., Proceedings of the National Academy of Sciences of the United States of America, 2007. [PMID=17483454]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G55740 Chloroplast
Plastid
ndhD 383 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0crr21-3T-DNA InsertionThe T-DNA insertion in crr21-3 is immediately upstream of the PG box and truncates CRR21 with loss of the PG boxNANANANABulk sequencingNAUneditedAbsent24194514
Col-0WTControlControlControlNormalNANABulk sequencingNACompleteNone24194514
Columbiacrr21-1Ds insertionDs is inserted in the CDS regionHeterozygousDefective NDH activityLeaf3 weeksDirect Sequencing of RT-PCR Products0.00%UneditedAbsent17483454
Columbiacrr21-2Ds insertionDs is inserted in the CDS regionHeterozygousDefective NDH activityLeaf3 weeksDirect Sequencing of RT-PCR Products0.00%UneditedAbsent17483454
ColumbiaWTControlControlControlNormalLeaf3 weeksDirect Sequencing of RT-PCR Products99.00%HighNone17483454
Columbiacrr21-1+CRR21ComplementationWild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-1NARestored the transient increase in chlorophyll fluorescence after AL illuminationLeaf3 weeksDirect Sequencing of RT-PCR Products97.00%HighRestored17483454
Columbiacrr21-2+CRR21ComplementationWild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-2NARestored the transient increase in chlorophyll fluorescence after AL illuminationLeaf3 weeksDirect Sequencing of RT-PCR Products100.00%CompleteRestored17483454
Col-0WTControlControlControlNANANARNA-seq96.67%HighNone32098170
Last update: Feb 2026 (version 2.0)