CRR21 - Plant Editosome Database - CNCB-NGDC

CRR21 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	CRR21
Synonym:	Chlororespiratory Reduction 21
Description:	The PG box of CRR21 is essential for editing ndhD C383; In crr21, the activity of the NDH complex is specifically impaired, suggesting that the Ser128Leu change has important consequences for the function of the NDH complex; E/E+ domain is highly conserved and exchangeable between CRR21 and CRR4, although it is not essential for the RNA binding
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E-E+
Gene ID & Species:	AT5G55740 (Arabidopsis thaliana)
Edited Gene(s):	ndhD
Editing Type(s):	C-to-U (7)
Publication(s):	[1] Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains, The Journal of Biological Chemistry, 2013. [doi:10.1074/jbc.M113.485755] [2] Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing, PNAS, 2007. [doi:10.1073/pnas.0700865104]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G55740

Chloroplast

383

CDS

NA

NA

NA

NA

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	crr21-3	T-DNA Insertion	The T-DNA insertion in crr21-3 is immediately upstream of the PG box and truncates CRR21 with loss of the PG box	NA	NA	NA	NA	Bulk sequencing	NA	Unedited	Absent	24194514
Col-0	WT	Control	Control	No mutant	Normal	NA	NA	Bulk sequencing	NA	Complete	None	24194514
Columbia	crr21-1	Ds insertion	Ds is inserted in the CDS region	Heterozygous	Defective NDH activity	Leaf	3 weeks	Direct Sequencing of RT-PCR Products	0.00%	Unedited	Absent	17483454
Columbia	crr21-2	Ds insertion	Ds is inserted in the CDS region	Heterozygous	Defective NDH activity	Leaf	3 weeks	Direct Sequencing of RT-PCR Products	0.00%	Unedited	Absent	17483454
Columbia	WT	Control	Control	No mutant	Normal	Leaf	3 weeks	Direct Sequencing of RT-PCR Products	99.00%	High	None	17483454
Columbia	crr21-1+CRR21	Complementation	Wild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-1	NA	Restored the transient increase in chlorophyll fluorescence after AL illumination	Leaf	3 weeks	Direct Sequencing of RT-PCR Products	97.00%	High	Restored	17483454
Columbia	crr21-2+CRR21	Complementation	Wild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-2	NA	Restored the transient increase in chlorophyll fluorescence after AL illumination	Leaf	3 weeks	Direct Sequencing of RT-PCR Products	100.00%	Complete	Restored	17483454

Last update: Sep 2026 (version 2.0)