CRR22 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

ndhB

746

CDS

Arabidopsis Genome Initiative
NA
EnsemblGenomes (release 39)

AP000423
NA
BK010421

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nossen	crr22-1	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△DYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△E/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	Nossen	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	93.52%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

ndhD

887

CDS

Arabidopsis Genome Initiative
NA
EnsemblGenomes (release 39)

AP000423
NA
BK010421

C-to-U

CCC=>CUC
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nossen	crr22-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△DYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△E/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	Nossen	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	82.87%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	66.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	84.00%	High	Increased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	96.00%	High	Increased	28761003

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

rpoB

551

CDS

Arabidopsis Genome Initiative
NA
EnsemblGenomes (release 39)

AP000423
NA
BK010421

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nossen	crr22-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△DYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nossen	crr22-1+CRR22△E/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nossen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nossen	Nossen	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	82.62%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	93.00%	High	Similar	28761003

Editing Factor:	CRR22
Synonym:	CHLORORESPIRATORY REDUCTION22
Description:	Encoding a PPR-DYW protein required for editing of multiple plastid transcripts
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT1G11290 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD rpoB
Editing Type(s):	C-to-U (39)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids., Nucleic acids research, 2012. [PMID=22362750] [4] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts., The Plant cell, 2009. [PMID=19182104]

Summary

Editing Details