CWM1 - Plant Editosome Database - CNCB-NGDC

CWM1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	CWM1
Synonym:	CELL WALL MAINTAINER 1
Description:	In addition to mutations in CELLULOSE SYNTHASE1 (CESA1) and CESA3, a forward genetic screen identified two independent defective genes encoding pentatricopeptide repeat (PPR)-like proteins (CELL WALL MAINTAINER1 [CWM1] and CWM2) as conferring tolerance to C17; Functional analysis revealed that mutations in these PPR proteins resulted in defective cytochrome c maturation and activation of mitochondrial retrograde signaling, as evidenced by the induction of an alternative oxidase; These mitochondrial perturbations increased tolerance to cell wall damage induced by cellulose deficiency; CWM1 also edits sites ccmCeU463RC, ccmBeU428SL, and nad5eU609VV; Surprisingly, the P. patens editing factor edited all target sites when expressed in the Arabidopsis cwm1 mutant background, even when carboxy-terminally truncated; Conversely, neither Arabidopsis CWM1 nor CWM1-PPR79 chimeras restored editing in P. patens ppr79 knockout plants
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2
Gene ID & Species:	NA (Macadamia integrifolia)
Edited Gene(s):	nad5 ccmB ccmC
Editing Type(s):	C-to-U (4)
Publication(s):	[1] One C-to-U RNA Editing Site and Two Independently Evolved Editing Factors: Testing Reciprocal Complementation with DYW-Type PPR Proteins from the Moss Physcomitrium (Physcomitrella) patens and the Flowering Plants Macadamia integrifolia and Arabidopsis, Plant Cell, 2020. [doi:10.1105/tpc.20.00311]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Macadamia integrifolia

NA

Mitochondrion

428

CDS

Pp3c5_7610V3.1 (P. patens nuclear genome v3.3)

NA

NA

NA

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	M. integrifolia CWM1	Control	Control	No mutant	NA	NA	NA	cDNA sequencing by Macrogen Europe, and peak height calculat	41.00%	Medium	None	32616665

Macadamia integrifolia

NA

Mitochondrion

463

CDS

Pp3c5_7610V3.1 (P. patens nuclear genome v3.3)

NA

NA

NA

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	M. integrifolia CWM1	Control	Control	No mutant	NA	NA	NA	cDNA sequencing by Macrogen Europe, and peak height calculat	100.00%	Complete	None	32616665

Macadamia integrifolia

NA

Mitochondrion

598

CDS

Pp3c5_7610V3.1 (P. patens nuclear genome v3.3)

NA

NA

NA

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	M. integrifolia CWM1	Control	Control	No mutant	NA	NA	NA	cDNA sequencing by Macrogen Europe, and peak height calculat	100.00%	Complete	None	32616665

Macadamia integrifolia

NA

Mitochondrion

609

CDS

Pp3c5_7610V3.1 (P. patens nuclear genome v3.3)

NA

NA

NA

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	M. integrifolia CWM1	Control	Control	No mutant	NA	NA	NA	cDNA sequencing by Macrogen Europe, and peak height calculat	0.00%	Unedited	None	32616665

Last update: Sep 2026 (version 2.0)