DEK10 - Plant Editosome Database

Editing Factor:	DEK10
Synonym:	Defective Kernel 10
Description:	Encodes an E-subgroup PPR protein localized in mitochondria. Sequencing analysis indicated that Dek10 is responsible for the C-to-U editing at nad3-61, nad3-62, and cox2-550 sites, which are specific editing sites in monocots.
Protein Family:	PPR
Subclass:	E
Construct Structure:	PLS-E
Gene ID & Species:	GRMZM2G087226 (Zea mays subsp. mays)
Edited Gene(s):	cox2 nad3
Editing Type(s):	C-to-U (9)
Publication(s):	[1] Editing of Mitochondrial Transcripts nad3 and cox2 by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development, Genetics, 2017. [PMID=28213476]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays subsp. mays

GRMZM2G087226

Mitochondrion

550

CDS

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	0.00%	Unedited	Absent	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	0.00%	Unedited	Absent	28213476
W22	WT	No treatment	No treatment	No treatment	No treatment	Kernel	18 DAP	Bulked sequencing	100.00%	Complete	None	28213476

Zea mays subsp. mays

GRMZM2G087226

Mitochondrion

CDS

C-to-U

CCA/CUA=>UCA/UUA

P/L=>S/L

Recoding/Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
W22	WT	No treatment	No treatment	No treatment	No treatment	Kernel	18 DAP	Bulked sequencing	70.00%	High	None	28213476

Zea mays subsp. mays

GRMZM2G087226

Mitochondrion

CDS

C-to-U

CCA/UCA=>CUA/UUA

P/S=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
W22	WT	No treatment	No treatment	No treatment	No treatment	Kernel	18 DAP	Bulked sequencing	75.00%	High	None	28213476