DEK10 - Plant Editosome Database

Summary

Editing Factor:	DEK10
Synonym:	Defective Kernel 10
Description:	Encoding an E-subgroup PPR protein localized in mitochondria. Sequencing analysis indicated that Dek10 is responsible for the C-to-U editing at nad3-61, nad3-62, and cox2-550 sites, which are specific editing sites in monocots.
Protein Family:	PPR
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	GRMZM2G087226 (Zea mays)
Edited Gene(s):	cox2 nad3
Editing Type(s):	C-to-U (9)
Publication(s):	[1] Editing of Mitochondrial Transcripts and by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development., Genetics, 2017. [PMID=28213476]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G087226

Mitochondrion

cox2

550

CDS

B73

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	0.00%	Unedited	Absent	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	0.00%	Unedited	Absent	28213476
W22	WT	Control	Control	Control	Normal	Kernel	18 DAP	Bulked sequencing	100.00%	Complete	None	28213476

Zea mays

GRMZM2G087226

Mitochondrion

nad3

CDS

B73

C-to-U

CCA/CUA=>UCA/UUA

P/L=>S/L

Recoding/Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
W22	WT	Control	Control	Control	Normal	Kernel	18 DAP	Bulked sequencing	70.00%	High	None	28213476

Zea mays

GRMZM2G087226

Mitochondrion

nad3

CDS

B73

C-to-U

CCA/UCA=>CUA/UUA

P/S=>L/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	dek10-Cas9	Multiplex CRISPR/Cas9 System	NA	NA	NA	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
dek10-N1176A	dek10-ref	Point Mutation	The mutation in the dek10-ref allele results in a premature termination in the first P motif	NA	Small kernels and delayed development	Kernel	18 DAP	Bulked sequencing	45.00%	Medium	Decreased	28213476
W22	WT	Control	Control	Control	Normal	Kernel	18 DAP	Bulked sequencing	75.00%	High	None	28213476