Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| W22 | WT | Wild Type | Wild Type | No mutant | Normal | Kernel | 12 DAP | RT‐PCR and sequencing | 93.50% | High | None | 32559332 |
| W22 | dek46-1 | Mutation | Dek46 contains one intron, in which a Mu element is inserted at +1471 bp (“A” of the start codon is assigned “+1”) in dek46‐1 | Recessive | The dek46‐1 mutant showed a defective kernel phenotype, with small kernels and shrunken pericarps after maturation. The volume of starchy endosperm in the mutant kernels is significantly lower than that in the wild type. The embryo is small and does not possess a scutellum, shoot apex or primary root primordium after maturation. | Kernel | 12 DAP | RT‐PCR and sequencing | 0.00% | Unedited | Absent | 32559332 |
| W22 | dek46-2 | Mutation | Dek46 contains one intron, in which a Mu element is inserted at +66 bp (“A” of the start codon is assigned “+1”) in dek46‐2 | Recessive; Heterozygous | Selfed heterozygous dek46‐2 plants segregated with a dek phenotype similar to that of dek46‐1. The dek46‐1 mutant showed a defective kernel phenotype, with small kernels and shrunken pericarps after maturation. The volume of starchy endosperm in the mutant kernels is significantly lower than that in the wild type. The embryo is small and does not possess a scutellum, shoot apex or primary root primordium after maturation. | Kernel | 12 DAP | RT‐PCR and sequencing | 0.00% | Unedited | Absent | 32559332 |
| W22 | WT | Wild Type | Wild Type | No mutant | Normal | Kernel | 12 DAP | RT‐PCR and sequencing | 81.93% | High | None | 32559332 |
| W22 | dek46-1 | Mutation | Dek46 contains one intron, in which a Mu element is inserted at +1471 bp (“A” of the start codon is assigned “+1”) in dek46‐1 | Recessive | The dek46‐1 mutant showed a defective kernel phenotype, with small kernels and shrunken pericarps after maturation. The volume of starchy endosperm in the mutant kernels is significantly lower than that in the wild type. The embryo is small and does not possess a scutellum, shoot apex or primary root primordium after maturation. | Kernel | 12 DAP | RT‐PCR and sequencing | 0.00% | Unedited | Absent | 32559332 |
| W22 | dek46-2 | Mutation | Dek46 contains one intron, in which a Mu element is inserted at +66 bp (“A” of the start codon is assigned “+1”) in dek46‐2 | Recessive; Heterozygous | Selfed heterozygous dek46‐2 plants segregated with a dek phenotype similar to that of dek46‐1. The dek46‐1 mutant showed a defective kernel phenotype, with small kernels and shrunken pericarps after maturation. The volume of starchy endosperm in the mutant kernels is significantly lower than that in the wild type. The embryo is small and does not possess a scutellum, shoot apex or primary root primordium after maturation. | Kernel | 12 DAP | RT‐PCR and sequencing | 0.00% | Unedited | Absent | 32559332 |