DEK504 - Plant Editosome Database

Editing Factor:	DEK504
Synonym:	Defective Kernel 504
Description:	Dek504 encodes an E+-type PPR protein targeted to the mitochondria, which is required for RNA editing of mitochondrial NADH dehydrogenase 3 at the nad3-317 and nad3-44 sites; Biochemical analysis of mitochondrial protein complexes revealed a significant reduction in the mitochondrial NADH dehydrogenase complex I activity, indicating that the alteration of the amino acid sequence at nad3-44 and nad3-317 through RNA editing is essential for NAD3 function
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	Zm00001d022394 (Zea mays)
Edited Gene(s):	nad3
Editing Type(s):	C-to-U (4)
Publication(s):	[1] Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize., International journal of molecular sciences, 2022. [PMID=35269656]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

Zm00001d022394

Mitochondrion

317

CDS

B73 (AGPv4)

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT	Control	Control	No mutant	Normal	Seed	15 DAP	Sequencing PCR products	100.00%	Complete	None	35269656
B73	dek504-ref	Mutants dek504-ref is derived from the inbred lines 14d3147	NA	Recessive; Heterozygous	Delayed embryogenesis and endosperm development, which produce small and collapsed kernels.	Seed	15 DAP	Sequencing PCR products	7.64%	Poor	Decreased	35269656