ECD1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: ECD1
Synonym: EMB2261, EMBRYO DEFECTIVE 2261
Description: Encodes an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G49170 (Arabidopsis thaliana)
Edited Gene(s): rps14
Editing Type(s): C-to-U (2)
Publication(s): [1] ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for earlychloroplast development in seedlings, Journal of Experimental Botany, 2018. [PMID=29648606]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G49170 Chloroplast rps14 149 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColumbiaWTNo treatmentNo treatmentNo treatmentNo treatmentCotyledon14 daysDirect Sequencing of RT-PCR Products91.00%HighNone29648606
ColumbiaECD1-RNAi-1RNAiA fragment of 434 bp of the ECD1 gene (from nucleotides 286 to 719) is amplifed and inserted into the PFGC5941 vector to generate RNAi constructKnockdownSeedlings with albino cotyledons but normal true leavesCotyledon14 daysDirect Sequencing of RT-PCR Products6.00%PoorRestored29648606
Last update: Jul 2021 (version 1.0)