EMP14 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

Zm00001d019525

Mitochondrion

ccmFc

966

CDS

B73 reference genome (RefGen V4)

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Wild type	No treatment	No mutant	Normal	Kernel	NA	The RT-PCR products were sequenced directly	100.00%	Complete	None	NONE
W22	emp14-ref	A single-base T insertion	A single-base thymine (T) insertion in the Zm00001d019525 gene at +831 bp downstream from the translation start codon ATG, resulting in a frame-shift in the open reading frame	Heterozygous; Recessive	The mutant emp14-ref kernels exhibited a reduction in size and were white in a segregating ear at 15 d after pollination (DAP); Sectioning of the developing seeds showed that the embryo and endosperm of emp14-ref were much smaller than those of WT; At 9 DAP, the WT embryos had advanced to the coleoptilar stage, while the emp14-ref embryos remained at the pre-embryo stage; At 12 DAP, the WT embryos had developed two leaf primordia and a root apical meristem, whereas the emp14-ref embryos remained at the transition stage; By 15 DAP, the WT embryos had advanced to the late embryogenesis stage, while the emp14-ref embryos remained at the transition stage	Kernel	NA	The RT-PCR products were sequenced directly	0.00%	Unedited	Absent	NONE

Zea mays

Zm00001d019525

Mitochondrion

nad4

819

CDS

B73 reference genome (RefGen V4)

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Wild type	No treatment	No mutant	Normal	Kernel	NA	The RT-PCR products were sequenced directly	58.00%	Medium	None	NONE
W22	emp14-ref	A single-base T insertion	A single-base thymine (T) insertion in the Zm00001d019525 gene at +831 bp downstream from the translation start codon ATG, resulting in a frame-shift in the open reading frame	Heterozygous; Recessive	The mutant emp14-ref kernels exhibited a reduction in size and were white in a segregating ear at 15 d after pollination (DAP); Sectioning of the developing seeds showed that the embryo and endosperm of emp14-ref were much smaller than those of WT; At 9 DAP, the WT embryos had advanced to the coleoptilar stage, while the emp14-ref embryos remained at the pre-embryo stage; At 12 DAP, the WT embryos had developed two leaf primordia and a root apical meristem, whereas the emp14-ref embryos remained at the transition stage; By 15 DAP, the WT embryos had advanced to the late embryogenesis stage, while the emp14-ref embryos remained at the transition stage	Kernel	NA	The RT-PCR products were sequenced directly	28.00%	Low	Decreased	NONE

Zea mays

Zm00001d019525

Mitochondrion

nad4

Intron

B73 reference genome (RefGen V4)

Intron 3

2687

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Wild type	No treatment	No mutant	Normal	Kernel	NA	The RT-PCR products were sequenced directly	61.00%	High	None	NONE
W22	emp14-ref	A single-base T insertion	A single-base thymine (T) insertion in the Zm00001d019525 gene at +831 bp downstream from the translation start codon ATG, resulting in a frame-shift in the open reading frame	Heterozygous; Recessive	The mutant emp14-ref kernels exhibited a reduction in size and were white in a segregating ear at 15 d after pollination (DAP); Sectioning of the developing seeds showed that the embryo and endosperm of emp14-ref were much smaller than those of WT; At 9 DAP, the WT embryos had advanced to the coleoptilar stage, while the emp14-ref embryos remained at the pre-embryo stage; At 12 DAP, the WT embryos had developed two leaf primordia and a root apical meristem, whereas the emp14-ref embryos remained at the transition stage; By 15 DAP, the WT embryos had advanced to the late embryogenesis stage, while the emp14-ref embryos remained at the transition stage	Kernel	NA	The RT-PCR products were sequenced directly	0.00%	Unedited	Absent	NONE
B73	emp14-2	A mutation	An EMS-induced G-to-A mutation at +512 bp, causing an alteration from TGG (Trp/W) to TAG (stop codon), resulting in a truncation of 170 amino acid residues	NA	An empty pericarp phenotype	Kernel	NA	The RT-PCR products were sequenced directly	0.00%	Unedited	Absent	NONE

Editing Factor:	EMP14
Synonym:	Empty Pericarp 14
Description:	The emp14 mutants were impaired in the C-to-U editing at nad4-i3-2687, nad4-819, and ccmFC-966 sites and the splicing of nad4 intron 3, resulting in substantially decreased nad4 transcript level and severely reduced assembly and activity of mitochondrial complex I; Loss of EMP14 function arrested embryogenesis and endosperm development, leaving an empty pericarp phenotype in maize
Protein Family:	Pentatricopeptide repeat (PPR)
Subclass:	E
Physical Interaction:	PCW1
Construct Structure:	PLS-E1-E2
Gene ID & Species:	Zm00001d019525 (Zea mays)
Edited Gene(s):	nad4 ccmFc
Editing Type(s):	C-to-U (7)
Publication(s):	NA

Summary

Editing Details