EMP9 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: EMP9
Synonym: Empty Pericarp 9; GROWING SLOWLY 1; GRS1
Description: Deletions of two N-terminal PPR motifs and partial E+ domain do not eliminate the editing function of EMP9; Loss of EMP9 function abolishes the C-to-U editing of ccmB-43 and rps4-335 sites in mitochondria; The loss of editing at ccmB-43 and rps4-335 affects the maturation of cytochrome c and impairs the biogenesis of mitochondrial respiratory complexes, particularly complex III
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: E+
Physical Interaction: NA
Construct Structure: PLS-E1-E2-E+
Gene ID & Species: AT4G32430 (Arabidopsis thaliana)
Edited Gene(s): rps4
Editing Type(s): C-to-U (4)
Publication(s): [1] The pentatricopeptide repeat protein EMP9 is required for mitochondrial ccmB and rps4 transcript editing, mitochondrial complex biogenesis and seed development in maize, New Phytologist, 2017. [doi:10.1111/nph.14424]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G32430 Mitochondrion rps4 377 CDS W22 NA NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
CS428796atemp9T-DNA InsertionNAKnockoutSeed and seedling lethalNANADirect Sequencing of PCR Products0.00%UneditedAbsent28121385
CS428796atemp9:atemp9 AtEmp9-FL-OX-1 (FL, full-length; OX, overexpression)ComplementationThe atemp9 mutants are complemented with the full-length AtEMP9 coding region under the control of CaMV 35 promoterNASeedling growth is restored to the WT levelNANADirect Sequencing of PCR Products100.00%CompleteRestored28121385
CS428796atemp9:atemp9 ZmEmp9-FL-OX-1 (FL, full-length; OX, overexpression)ComplementationThe full-length EMP9 coding region is expressed in the atemp9 plants under the control of the CaMV 35S promoterNAGrow wellNANADirect Sequencing of PCR Products30.00%LowRestored28121385
Col-0WTControlControlNo mutantNormalNANADirect Sequencing of PCR Products100.00%CompleteNone28121385
Last update: Sep 2026 (version 2.0)