EMP9 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: EMP9
Synonym: Empty Pericarp 9; GROWING SLOWLY 1; GRS1
Description: Deletions of two N-terminal PPR motifs and partial E+ domain do not eliminate the editing function of EMP9; Loss of EMP9 function abolishes the C-to-U editing of ccmB-43 and rps4-335 sites in mitochondria; The loss of editing at ccmB-43 and rps4-335 affects the maturation of cytochrome c and impairs the biogenesis of mitochondrial respiratory complexes, particularly complex III
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: E+
Physical Interaction: NA
Construct Structure: PLS-E1-E2-E+
Gene ID & Species: AC212684.3_FG012 (Zea mays)
Edited Gene(s): ccmB    rps4
Editing Type(s): C-to-U (16)
Publication(s): [1] The pentatricopeptide repeat protein EMP9 is required for mitochondrial ccmB and rps4 transcript editing, mitochondrial complex biogenesis and seed development in maize, New Phytologist, 2017. [doi:10.1111/nph.14424]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays AC212684.3_FG012 Mitochondrion ccmB 43 CDS W22 NA NA NA C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
W22emp9-1Mu InsertionMu is inserted in the middle of the PPR repeatsRecessive; HeterozygousArrested in embryogenesis and endosperm development; The mutant kernels are white, small and defectiveKernelNADirect Sequencing of PCR Products0.00%UneditedAbsent28121385
NAemp9-2Mu InsertionMu is inserted in the E domain (at +2074 bp)HeterozygousSevere emp phenotypeKernelNADirect Sequencing of PCR Products0.00%UneditedAbsent28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel9 DAPDirect Sequencing of PCR Products25.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel11 DAPDirect Sequencing of PCR Products25.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel14 DAPDirect Sequencing of PCR Products25.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeLeafNADirect Sequencing of PCR Products25.00%LowDecreased28121385
NAemp9-4Mu InsertionMu is inserted in the E+ domain (at +2190 bp)NANo visible seed and seedling phenotypesKernelNADirect Sequencing of PCR Products75.00%HighDecreased28121385
NACol-0ControlControlNo mutantNormalKernelNADirect Sequencing of PCR Products90.00%HighNone28121385
Zea mays AC212684.3_FG012 Mitochondrion rps4 335 CDS W22 NA NA NA C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAemp9-1Mu InsertionMu is inserted in the middle of the PPR repeatsRecessive; HeterozygousArrested in embryogenesis and endosperm development; The mutant kernels are white, small and defectiveKernelNADirect Sequencing of PCR Products0.00%UneditedAbsent28121385
NAemp9-2Mu InsertionMu is inserted in the E domain (at +2074 bp)HeterozygousSevere emp phenotypeKernelNADirect Sequencing of PCR Products0.00%UneditedAbsent28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel9 DAPDirect Sequencing of PCR Products20.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel11 DAPDirect Sequencing of PCR Products20.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeKernel14 DAPDirect Sequencing of PCR Products20.00%LowDecreased28121385
NAemp9-3Mu InsertionMu is inserted in the putative N-terminal signal peptide (sp) region (at +19 bp)NASlow growth (Slo) phenotypeLeafNADirect Sequencing of PCR Products20.00%LowDecreased28121385
NAemp9-4Mu InsertionMu is inserted in the E+ domain (at +2190 bp)NANo visible seed and seedling phenotypesKernelNADirect Sequencing of PCR Products75.00%HighDecreased28121385
NACol-0ControlControlNo mutantNormalKernelNADirect Sequencing of PCR Products90.00%HighNone28121385
Last update: Sep 2026 (version 2.0)