Plant Editosome Database
a curated database of RNA editosome in plants

GEND2 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: GEND2
Synonym: NA
Description: A T-DNA insertion in the 5′-untranslated region of GEND2 disrupts mitochondrial RNA editing, leading to mitochondrial dysfunction; This dysfunction triggers an ANAC017-dependent signaling pathway to counteract cell wall damage induced by cellulose synthase inhibitors, as well as an ANAC017-independent pathway that impairs root growth under normal conditions
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: AT5G39680 (Arabidopsis thaliana)
Edited Gene(s): ccmFn-1    rpsl2    orfX
Editing Type(s): C-to-U (24)
Publication(s): [1] Arabidopsis Pentatricopeptide Repeat Protein GEND2 Participates in Mitochondrial RNA Editing., Plant & cell physiology, 2024. [PMID=39301683]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G39680 Mitochondrion ccmFn-1 791 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK494.00%HighNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK42.00%PoorDecreased39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK481.00%HighRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK485.00%HighRestored39301683
Arabidopsis thaliana AT5G39680 Mitochondrion ccmFn-1 955 CDS C-to-U CGC=>UGC R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK4100.00%CompleteNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK40.00%UneditedAbsent39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK490.00%HighRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK492.00%HighRestored39301683
Arabidopsis thaliana AT5G39680 Mitochondrion orfX 361 CDS C-to-U CUC=>UUC L=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK492.00%HighNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK465.00%HighDecreased39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK4100.00%CompleteRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK489.00%HighRestored39301683
Arabidopsis thaliana AT5G39680 Mitochondrion orfX 364 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK488.00%HighNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK463.00%HighDecreased39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK4100.00%CompleteRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK483.00%HighRestored39301683
Arabidopsis thaliana AT5G39680 Mitochondrion orfX 59 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK477.00%HighNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK40.00%UneditedAbsent39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK489.00%HighRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK495.00%HighRestored39301683
Arabidopsis thaliana AT5G39680 Mitochondrion rpsl2 146 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)WTControlControlNo mutantNormalSeedlingNASanger sequencing, GATK441.00%MediumNone39301683
NAgend2-1A T-DNA insertionA T-DNA insertion in the 5′-untranslated regionHomozygousgend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutantsSeedlingNASanger sequencing, GATK411.00%PoorDecreased39301683
NAComp-1ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK444.00%MediumRestored39301683
NAComp-2ComplementationA genomic DNA fragment of AT5G39680 into the gend2-1 mutantHomozygousThe full recovery of primary root and RAM defects in gend2-1SeedlingNASanger sequencing, GATK440.00%MediumRestored39301683
Last update: Jul 2021 (version 1.0)