GEND2 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G39680

Mitochondrion

ccmFn-1

791

CDS

TAIR10

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	94.00%	High	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	2.00%	Poor	Decreased	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	81.00%	High	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	85.00%	High	Restored	39301683

Arabidopsis thaliana

AT5G39680

Mitochondrion

ccmFn-1

955

CDS

TAIR10

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	100.00%	Complete	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	0.00%	Unedited	Absent	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	90.00%	High	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	92.00%	High	Restored	39301683

Arabidopsis thaliana

AT5G39680

Mitochondrion

orfX

361

CDS

TAIR10

C-to-U

CUC=>UUC

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	92.00%	High	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	65.00%	High	Decreased	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	100.00%	Complete	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	89.00%	High	Restored	39301683

Arabidopsis thaliana

AT5G39680

Mitochondrion

orfX

364

CDS

TAIR10

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	88.00%	High	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	63.00%	High	Decreased	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	100.00%	Complete	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	83.00%	High	Restored	39301683

Arabidopsis thaliana

AT5G39680

Mitochondrion

orfX

CDS

TAIR10

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	77.00%	High	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	0.00%	Unedited	Absent	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	89.00%	High	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	95.00%	High	Restored	39301683

Arabidopsis thaliana

AT5G39680

Mitochondrion

rpsl2

146

CDS

TAIR10

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	NA	Sanger sequencing, GATK4	41.00%	Medium	None	39301683
NA	gend2-1	A T-DNA insertion	A T-DNA insertion in the 5′-untranslated region	Homozygous	gend2-1 seedlings exhibited primary roots significantly shorter than their wild-type (WT) counterparts; The gend2-1 mutants displayed noticeably shorter RAM and fewer meristematic cells; After subjecting the seedlings to EdU solution for a period of 3 h, WT plants exhibited a higher number of EdU-positive cells in the primary root compared to gend2-1 mutants	Seedling	NA	Sanger sequencing, GATK4	11.00%	Poor	Decreased	39301683
NA	Comp-1	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	44.00%	Medium	Restored	39301683
NA	Comp-2	Complementation	A genomic DNA fragment of AT5G39680 into the gend2-1 mutant	Homozygous	The full recovery of primary root and RAM defects in gend2-1	Seedling	NA	Sanger sequencing, GATK4	40.00%	Medium	Restored	39301683

Editing Factor:	GEND2
Synonym:	NA
Description:	A T-DNA insertion in the 5′-untranslated region of GEND2 disrupts mitochondrial RNA editing, leading to mitochondrial dysfunction; This dysfunction triggers an ANAC017-dependent signaling pathway to counteract cell wall damage induced by cellulose synthase inhibitors, as well as an ANAC017-independent pathway that impairs root growth under normal conditions
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	AT5G39680 (Arabidopsis thaliana)
Edited Gene(s):	ccmFn-1 rpsl2 orfX
Editing Type(s):	C-to-U (24)
Publication(s):	[1] Arabidopsis Pentatricopeptide Repeat Protein GEND2 Participates in Mitochondrial RNA Editing., Plant & cell physiology, 2024. [PMID=39301683]

Summary

Editing Details