GRS1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad1

265

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad1

265

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.05%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	11.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad4L

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad4L

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	94.28%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	17.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad6

103

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad6

103

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	98.34%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	98.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	1.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

rps4

377

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

rps4

377

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	87.31%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	2.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Editing Factor:	GRS1
Synonym:	GROWING SLOWLY 1
Description:	GRS1 is a mitochondrial PLS-type PRR protein required for RNA editing and plant development
Protein Family:	PPR
Subclass:	DYW,E+
Construct Structure:	PLS-E1-E2-DYW, NA
Gene ID & Species:	NA (Arabidopsis thaliana) AT4G32430 (Arabidopsis thaliana)
Edited Gene(s):	nad1 nad4L nad6 rps4
Editing Type(s):	C-to-U (32)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Growing Slowly 1 locus encodes a PLS-type PPR protein required for RNA editing and plant development in Arabidopsis., Journal of experimental botany, 2016. [PMID=27670716]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details