GRS1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad1

265

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad1

265

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.05%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	11.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad4L

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad4L

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	94.28%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	17.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad6

103

CDS

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

nad6

103

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	98.34%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	98.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	1.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

rps4

377

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	Control	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716

Arabidopsis thaliana

Mitochondrion

rps4

377

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	87.31%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	2.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Editing Factor:	GRS1
Synonym:	GROWING SLOWLY 1
Description:	GRS1 is a mitochondrial PLS-type PRR protein required for RNA editing and plant development
Protein Family:	PPR
Subclass:	DYW,E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW, NA
Gene ID & Species:	NA (Arabidopsis thaliana) AT4G32430 (Arabidopsis thaliana)
Edited Gene(s):	nad1 nad4L nad6 rps4
Editing Type(s):	C-to-U (32)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Growing Slowly 1 locus encodes a PLS-type PPR protein required for RNA editing and plant development in Arabidopsis., Journal of experimental botany, 2016. [PMID=27670716]

Summary

Editing Details