GRS1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad1

265

CDS

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	No mutant	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	11.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad4L

CDS

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	No mutant	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	17.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

nad6

103

CDS

C-to-U

CGC=>UGC
NA=>NA

R=>C
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	No mutant	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	98.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	1.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT4G32430

Mitochondrion

rps4

377

CDS

C-to-U

CCG=>CUG
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	grs1-1	T-DNA Insertion	T-DNA is located at 1325 bp downstream of the ATG start codon	Knockout	grs1-1 homozygotes are either lethal or exhibited growth retardation; cell division activity is reduced dramatically in the roots of grs1 plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27670716
NA	grs1-2	T-DNA Insertion	T-DNA is inserted at 850 bp downstream of the ATG start codon	Knockout	Homozygous grs1-2 plants are found to phenocopy grs1-1 homozygous plants	Seedling	20 days	Direct Sequencing of PCR Products	0.00%	Unedited	None	27670716
qrt 1	WT	Control	Control	No mutant	Normal	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	None	27670716
NA	grs1-1 complemented lines	Complementation	Genomic complemented lines	Homozygous	No obvious differences compared to the wild-type	Seedling	20 days	Direct Sequencing of PCR Products	100.00%	Complete	Restored	27670716
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	2.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Editing Factor:	GRS1
Synonym:	GROWING SLOWLY 1
Description:	GRS1 is responsible for the RNA editing at four specific sites of four mitochondrial mRNAs: nad1-265, nad4L-55, nad6-103, and rps4-377 The first three of these mRNAs encode for the subunits of complex I of the electron transport chain in mitochondria; The activity of complex I is strongly reduced in grs1 Changes in RPS4 editing in grs1 plants affect mitochondrial ribosome biogenesis; Expression of the alternative respiratory pathway and the abscisic acid response gene ABI5 were up-regulated in grs1 mutant plants Genetic analysis revealed that ABI5 is involved in the short root phenotype of grs1
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	AT4G32430 (Arabidopsis thaliana)
Edited Gene(s):	nad1 nad4L nad6 rps4
Editing Type(s):	C-to-U (28)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] Growing Slowly 1 locus encodes a PLS-type PPR protein required for RNA editing and plant development in Arabidopsis, Journal of Experimental Botany, 2016. [doi:10.1093/jxb/erw331]

Summary

Editing Details