Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Zhonghuang 39 | EV | Control | Empty vector (EV)-transformed control hairy root; The coding sequences of GmPPR4 was ligated into plant transformation vector pCAMBIA3301 with the CaMV 35S promoter. The constructs were transferred in | No treatment | The EV-control plants showed wilted leaf under drought for 3 days, and seriously dehydrated leaves were observed after 7 days; Trypan blue solution was used to detect cell activity in EV-control and GmPPR4-OE leaves, no plant leaves differed under drought stress conditions, however, the color depth of the GmPPR4-OE leaves was lower than EV-control leaves under drought treatment. | Leaf | 15 days | NA | NA | NA | NA | 31726763 |
| Zhonghuang 39 | OE-1 | Overexpressed GmPPR4 | Transgenic soybean plants which overexpressed GmPPR4 (GmPPR4-OE) were generated into soybean hairy roots; The coding sequences of GmPPR4 was ligated into plant transformation vector pCAMBIA3301 with t | NA | The GmPPR4-OE seedlings showed delayed and less leaf rolling during the drought stress process. Trypan blue solution was used to detect cell activity in EV-control and GmPPR4-OE leaves, no plant leaves differed under drought stress conditions, however, the color depth of the GmPPR4-OE leaves was lower than EV-control leaves under drought treatment. | Leaf | 15 days | NA | NA | NA | NA | 31726763 |
| Zhonghuang 39 | OE-2 | Overexpressed GmPPR4 | Transgenic soybean plants which overexpressed GmPPR4 (GmPPR4-OE) were generated into soybean hairy roots; The coding sequences of GmPPR4 was ligated into plant transformation vector pCAMBIA3301 with t | NA | The GmPPR4-OE seedlings showed delayed and less leaf rolling during the drought stress process. Trypan blue solution was used to detect cell activity in EV-control and GmPPR4-OE leaves, no plant leaves differed under drought stress conditions, however, the color depth of the GmPPR4-OE leaves was lower than EV-control leaves under drought treatment. | Leaf | 15 days | NA | NA | NA | NA | 31726763 |