Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Dongfudou 3 | EV | Well-watered conditions; Empty-vector control | Control plants were maintained at approximately 80–85% field capacity | No mutant | Under well-watered conditions, the MDA content in KO-4 was significantly higher than in EV; Under well-watered conditions, POD activity in KO-4 was significantly lower than in EV; Drought stress significantly induced soluble sugar accumulation in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS | Root | NA | Sanger sequencing of the purified PCR product | 82.00% | High | None | 42519610 |
| Dongfudou 3 | EV-DS | Drought stress treatment; Empty-vector control | For drought treatment, irrigation was withheld until soil water content decreased to 50–55% field capacity on day 5, when plants showed mild drought symptoms | No mutant | Drought stress markedly increased MDA levels in both genotypes, with KO-4-DS consistently showing significantly higher MDA content than EV-DS under stress; Drought stress significantly induced POD activity in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS; Drought stress significantly induced soluble sugar accumulation in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS; Soluble sugar content in KO-4-DS remained significantly lower than in EV-DS under stress | Root | NA | Sanger sequencing of the purified PCR product | 22.00% | Low | None | 42519610 |
| Dongfudou 3 | KO-4 | Well-watered conditions; CRISPR knockout | Control plants were maintained at approximately 80–85% field capacity; The CRISPR/Cas9-mediated gene editing construct targeting Gm_DFD3_00451 was introduced into K599 for hairy root-mediated transfor | Knockout | Under well-watered conditions, the MDA content in KO-4 was significantly higher than in EV; Under well-watered conditions, POD activity in KO-4 was significantly lower than in EV; Drought stress significantly induced soluble sugar accumulation in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS | Root | NA | Sanger sequencing of the purified PCR product | 0.00% | Unedited | Absent | 42519610 |
| Dongfudou 3 | KO-4-DS | Drought stress treatment; CRISPR knockout | For drought treatment, irrigation was withheld until soil water content decreased to 50–55% field capacity on day 5, when plants showed mild drought symptoms; The CRISPR/Cas9-mediated gene editing con | Knockout | Drought stress markedly increased MDA levels in both genotypes, with KO-4-DS consistently showing significantly higher MDA content than EV-DS under stress; Drought stress significantly induced POD activity in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS; Drought stress significantly induced soluble sugar accumulation in both genotypes, but the increase in KO-4-DS was markedly smaller than that in EV-DS; Soluble sugar content in KO-4-DS remained significantly lower than in EV-DS under stress | Root | NA | Sanger sequencing of the purified PCR product | 0.00% | Unedited | Absent | 42519610 |
