LPA66 - Plant Editosome Database

Summary

Editing Factor:	LPA66
Synonym:	LOW PSII ACCUMULATION 66
Description:	The assembly of newly synthesized proteins into PSII occurs in the lpa66 mutant but with reduced efficiency compared with the wild type; LPA66 is specifically required for editing the psbF transcripts in Arabidopsis, and the amino acid alternation due to lack of editing strongly affects the efficiency of the assembly of PSII complexes
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT5G48910 (Arabidopsis thaliana)
Edited Gene(s):	psbF
Editing Type(s):	C-to-U (6)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] LPA66 is required for editing psbF chloroplast transcripts in Arabidopsis, Plant Physiology, 2009. [doi:10.1104/pp.109.136812]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G48910

Chloroplast

psbF

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
screened from a collection of pSKI015 T-DNAmutagenized Arabidopsis (Arabidopsis thaliana ecotype Columbia) lines	lpa66-1	Point Mutation	An amino acid is changed (415) from Gly to Arg	Homozygous	A high chlorophyll fluorescence phenotype; The mutant plants showed reduced growth; The leaves appeared pale green under optimal growth conditions	Leaf	5 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
CS813518	lpa66-2	T-DNA Insertion	T-DNA is inserted at nucleotide position 1,110 bp of the AT5G48910 gene relative to the ATG codon	NA	Pale green and indistinguishable from that of lpa66-1	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
NA	WT	Control	Control	No mutant	Normal	Leaf	5 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19448041
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	88.00%	High	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Similar	28761003