LPA66 - Plant Editosome Database - BIG Data Center

LPA66 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	LPA66
Synonym:	LOW PSII ACCUMULATION 66
Description:	Encodes a chloroplast protein of the pentatricopeptide repeat family. lpa66 mutants impair the editing of psbF that converts serine to phenylalanine and display a high chlorophyll fluorescence phenotype.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT5G48910 (Arabidopsis thaliana)
Edited Gene(s):	psbF
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] LPA66 is required for editing psbF chloroplast transcripts in Arabidopsis, Plant Physiology, 2009. [PMID=19448041]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G48910

Chloroplast
Plastid

77

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
screened from a collection of pSKI015 T-DNAmutagenized Arabidopsis (Arabidopsis thaliana ecotype Columbia) lines	lpa66-1	Point Mutation	An amino acid is changed (415) from Gly to Arg	The abundance of AT5G48910 transcripts in the lpa66-1 mutant was comparable to that in wild type plants; Recessive Mutant	A high chlorophyll fluorescence phenotype; The mutant plants showed reduced growth; The leaves appeared pale green under optimal growth conditions	Leaf	5 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
CS813518	lpa66-2	T-DNA Insertion	T-DNA is inserted at nucleotide position 1,110 bp of the AT5G48910 gene relative to the ATG codon	No Expression; Null Mutant	Pale green and indistinguishable from that of lpa66-1	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
NA	WT	No treatment	No treatment	No treatment	No treatment	Leaf	5 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19448041
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	97.52%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	88.00%	High	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Similar	28761003

Last update: Jul 2021 (version 1.0)