LPA66 - Plant Editosome Database

Summary

Editing Factor:	LPA66
Synonym:	LOW PSII ACCUMULATION 66
Description:	Encoding a chloroplast protein of the pentatricopeptide repeat family; lpa66 mutants impair the editing of psbF that converts serine to phenylalanine and display a high chlorophyll fluorescence phenotype.
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT5G48910 (Arabidopsis thaliana)
Edited Gene(s):	psbF
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] LPA66 is required for editing psbF chloroplast transcripts in Arabidopsis., Plant physiology, 2009. [PMID=19448041]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G48910

Chloroplast
Plastid

psbF

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
screened from a collection of pSKI015 T-DNAmutagenized Arabidopsis (Arabidopsis thaliana ecotype Columbia) lines	lpa66-1	Point Mutation	An amino acid is changed (415) from Gly to Arg	The abundance of AT5G48910 transcripts in the lpa66-1 mutant was comparable to that in wild type plants; Recessive Mutant	A high chlorophyll fluorescence phenotype; The mutant plants showed reduced growth; The leaves appeared pale green under optimal growth conditions	Leaf	5 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
CS813518	lpa66-2	T-DNA Insertion	T-DNA is inserted at nucleotide position 1,110 bp of the AT5G48910 gene relative to the ATG codon	No Expression; Null Mutant	Pale green and indistinguishable from that of lpa66-1	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19448041
NA	WT	Control	Control	Control	Normal	Leaf	5 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	19448041
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	97.52%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	88.00%	High	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Similar	28761003