MEF100 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G61170

Mitochondrion

ccmFn2

356

CDS

Genomic position

291,935

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Wild Type	Wild Type	No mutant	Normal	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	None	33671598
Columbia (Col-0)	mef100	Point mutation	The mef100 mutant previously isolated in a screen for BSO-insensitive Arabidopsis mutants contains an EMS-induced point mutation in the gene AT3G61170 that converts the 1497th nucleotide in the coding	Homozygous	A delayed growth rate; The mutant plants display a dark curly foliage typical for Complex I mutants	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	0.00%	Unedited	Absent	33671598
Columbia (Col-0)	Comp	Mutant Complementation	The region of genomic DNA corresponding to the full mef100 gene and 522 nt upstream of the mef100 start codon was amplified by PCR from Col-0 genomic DNA using mef100_ups_attB_F and mef100-stop_attBR,	Homozygous	The wild-type phenotype is completely restored in complemented plants expressing a functional copy of the MEF100 gene including 522 nucleotides upstream of the potential start codon.	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	Restored	33671598

Arabidopsis thaliana

AT3G61170

Mitochondrion

nad1

493

CDS

Genomic position

59,314

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Wild Type	Wild Type	No mutant	Normal	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	None	33671598
Columbia (Col-0)	mef100	Point mutation	The mef100 mutant previously isolated in a screen for BSO-insensitive Arabidopsis mutants contains an EMS-induced point mutation in the gene AT3G61170 that converts the 1497th nucleotide in the coding	Homozygous	A delayed growth rate; The mutant plants display a dark curly foliage typical for Complex I mutants	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	15.38%	Poor	Decreased	33671598
Columbia (Col-0)	Comp	Mutant Complementation	The region of genomic DNA corresponding to the full mef100 gene and 522 nt upstream of the mef100 start codon was amplified by PCR from Col-0 genomic DNA using mef100_ups_attB_F and mef100-stop_attBR,	Homozygous	The wild-type phenotype is completely restored in complemented plants expressing a functional copy of the MEF100 gene including 522 nucleotides upstream of the potential start codon.	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	Restored	33671598

Arabidopsis thaliana

AT3G61170

Mitochondrion

nad4

403

CDS

Genomic position

215,160

C-to-U

CGC=>UGC

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Wild Type	Wild Type	No mutant	Normal	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	None	33671598
Columbia (Col-0)	mef100	Point mutation	The mef100 mutant previously isolated in a screen for BSO-insensitive Arabidopsis mutants contains an EMS-induced point mutation in the gene AT3G61170 that converts the 1497th nucleotide in the coding	Homozygous	A delayed growth rate; The mutant plants display a dark curly foliage typical for Complex I mutants	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	0.24%	Poor	Decreased	33671598
Columbia (Col-0)	Comp	Mutant Complementation	The region of genomic DNA corresponding to the full mef100 gene and 522 nt upstream of the mef100 start codon was amplified by PCR from Col-0 genomic DNA using mef100_ups_attB_F and mef100-stop_attBR,	Homozygous	The wild-type phenotype is completely restored in complemented plants expressing a functional copy of the MEF100 gene including 522 nucleotides upstream of the potential start codon.	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	Restored	33671598

Arabidopsis thaliana

AT3G61170

Mitochondrion

nad7

698

CDS

Genomic position

241,553

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	Col-0	Wild Type	Wild Type	No mutant	Normal	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	None	33671598
Columbia (Col-0)	mef100	Point mutation	The mef100 mutant previously isolated in a screen for BSO-insensitive Arabidopsis mutants contains an EMS-induced point mutation in the gene AT3G61170 that converts the 1497th nucleotide in the coding	Homozygous	A delayed growth rate; The mutant plants display a dark curly foliage typical for Complex I mutants	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	0.00%	Unedited	Absent	33671598
Columbia (Col-0)	Comp	Mutant Complementation	The region of genomic DNA corresponding to the full mef100 gene and 522 nt upstream of the mef100 start codon was amplified by PCR from Col-0 genomic DNA using mef100_ups_attB_F and mef100-stop_attBR,	Homozygous	The wild-type phenotype is completely restored in complemented plants expressing a functional copy of the MEF100 gene including 522 nucleotides upstream of the potential start codon.	Leaf	8 weeks	Sanger sequencing of the RT-PCR products	100.00%	Complete	Restored	33671598

Editing Factor:	MEF100
Synonym:	Mitochondrial Editing Factor 100
Description:	A combined approach of RNA-Seq, mutant complementation, electrophoresis of mitochondrial protein complexes and Western blotting allowed us to show that MEF100, a PPR protein identified in a genetic screen for mutants resistant to an inhibitor of γ -glutamylcysteine synthetase, is required for the editing of nad1-493, nad4-403, nad7-698 and ccmFN2-356 sites in Arabidopsis mitochondria; The absence of editing in mef100 leads to a decrease in mitochondrial Complex I activity, which probably explains the physiological phenotype
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	AT3G61170 (Arabidopsis thaliana)
Edited Gene(s):	nad1 nad4 nad7 ccmFn2
Editing Type(s):	C-to-U (12)
Publication(s):	[1] The Pentatricopeptide Repeat Protein MEF100 Is Required for the Editing of Four Mitochondrial Editing Sites in ., Cells, 2021. [PMID=33671598]

Summary

Editing Details