MEF14 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF14
Synonym: MITOCHONDRIAL EDITING FACTOR 14
Description: Encodes a PPR (pentatricopeptide repeat proteins) protein required for RNA editing at site matR-1895 in mitochondria. The mRNA is cell-to-cell mobile.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G26780 (Arabidopsis thaliana)
Edited Gene(s): matR
Editing Type(s): C-to-U (8)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The DYW-E-PPR protein MEF14 is required for RNA editing at site matR-1895 in mitochondria of Arabidopsis thaliana, FEBS Letters, 2011. [PMID=21281638]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G26780 Mitochondrion matR 1895 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0CT(+At3g26780)ComplementationThe col wild type version of the MEF14 gene is introduced into mef14-1 mutant protoplastsNANAProtoplast3-4 weeksDirect Sequencing of PCR Products48.00%MediumDecreased21281638
Col-0mef14-1EMS MutantAn amino acid in the MEF14 RNA editing factor (Asp455Asn) is alteredRecessive alleleNormal growth phenotypeLeafNADirect Sequencing of PCR Products30.00%LowDecreased21281638
SALK_131077mef14-2T-DNA InsertionT-DNA is inserted in one of the central PPR repeatsHomozygous; KnockoutNormal growth phenotypeLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21281638
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone21281638
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq93.86%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)