MEF14 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MEF14
Synonym: MITOCHONDRIAL EDITING FACTOR 14
Description: Encoding a PPR (pentatricopeptide repeat proteins) protein required for RNA editing at site matR-1895 in mitochondria; The mRNA is cell-to-cell mobile.
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G26780 (Arabidopsis thaliana)
Edited Gene(s): matR
Editing Type(s): C-to-U (8)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The DYW-E-PPR protein MEF14 is required for RNA editing at site matR-1895 in mitochondria of Arabidopsis thaliana., FEBS letters, 2011. [PMID=21281638]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G26780 Mitochondrion matR 1895 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0CT(+At3g26780)ComplementationThe col wild type version of the MEF14 gene is introduced into mef14-1 mutant protoplastsNANAProtoplast3-4 weeksDirect Sequencing of PCR Products48.00%MediumDecreased21281638
Col-0mef14-1EMS MutantAn amino acid in the MEF14 RNA editing factor (Asp455Asn) is alteredRecessiveNormal growth phenotypeLeafNADirect Sequencing of PCR Products30.00%LowDecreased21281638
SALK_131077mef14-2T-DNA InsertionT-DNA is inserted in one of the central PPR repeatsHomozygous; KnockoutNormal growth phenotypeLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21281638
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone21281638
Col-0WTControlControlControlNANANARNA-seq93.86%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighSimilar28761003
Last update: Feb 2026 (version 2.0)