MEF14 - Plant Editosome Database

Summary

Editing Factor:	MEF14
Synonym:	MITOCHONDRIAL EDITING FACTOR 14
Description:	A T-DNA insertion in the MEF14 gene abolishes detectable editing at the matR-1895 site; Lack of RNA editing at the matR-1895 site does not alter the level of mature and precursor nad1 mRNA molecules; Such RNA editing mutants can be used to analyse the function of genes like this maturase related reading frame in plant mitochondria
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT3G26780 (Arabidopsis thaliana)
Edited Gene(s):	matR
Editing Type(s):	C-to-U (7)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The DYW-E-PPR protein MEF14 is required for RNA editing at site matR-1895 in mitochondria of Arabidopsis thaliana, FEBS Letters, 2011. [doi:10.1016/j.febslet.2011.01.037]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G26780

Mitochondrion

matR

1895

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	CT(+At3g26780)	Complementation	The col wild type version of the MEF14 gene is introduced into mef14-1 mutant protoplasts	NA	NA	Protoplast	3-4 weeks	Direct Sequencing of PCR Products	48.00%	Medium	Decreased	21281638
Col-0	mef14-1	EMS Mutant	An amino acid in the MEF14 RNA editing factor (Asp455Asn) is altered	Recessive	Normal growth phenotype	Leaf	NA	Direct Sequencing of PCR Products	30.00%	Low	Decreased	21281638
SALK_131077	mef14-2	T-DNA Insertion	T-DNA is inserted in one of the central PPR repeats	Homozygous; Knockout	Normal growth phenotype	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	21281638
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	21281638
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	93.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	94.00%	High	Similar	28761003