MEF18 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF18
Synonym: MITOCHONDRIAL EDITING FACTOR 18
Description: Encoding a pentatricopeptide repeat protein (PPR) protein involved in mitochondrial mRNA editing
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT5G19020 (Arabidopsis thaliana)
Edited Gene(s): nad4
Editing Type(s): C-to-U (6)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Reverse genetic screening identifies five E-class PPR proteins involved in RNA editing in mitochondria of Arabidopsis thaliana., The Journal of biological chemistry, 2010. [PMID=20566637]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G19020 Mitochondrion nad4 1355 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_126644Cmef18T-DNA InsertionT-DNA is inserted in the CDSHomozygousNo gross disturbance in their growth or development patternsLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent20566637
SALK_126644Cmef18ComplementationIntroduction of the Col wild type version of the gene MEF18 into the mutant protoplastsNANAProtoplastNADirect Sequencing of PCR ProductsNAHighRestored20566637
Col-0WTControlControlControlNANANARNA-seq99.74%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)