MEF22 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MEF22
Synonym: MITOCHONDRIAL EDITING FACTOR 22
Description: Encoding a pentatricopeptide repeat protein (PPR) protein involved in mitochondrial mRNA editing.
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-DYW
Gene ID & Species: AT3G12770 (Arabidopsis thaliana)
Edited Gene(s): nad3
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Reverse genetic screening identifies five E-class PPR proteins involved in RNA editing in mitochondria of Arabidopsis thaliana., The Journal of biological chemistry, 2010. [PMID=20566637]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G12770 Mitochondrion nad3 149 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCC=>UUC
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone20566637
SALK_107216Cmef22T-DNA InsertionT-DNA is inserted in the CDSHomozygousNo gross disturbance in their growth or development patternsLeafNADirect Sequencing of PCR Products60.00%HighDecreased20566637
SALK_107216Cmef22ComplementationIntroduction of the Col wild type version of the gene MEF22 into the mutant protoplastsNANAProtoplastNADirect Sequencing of PCR ProductsNAHighDecreased20566637
Col-0WTControlControlControlNANANARNA-seq75.77%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract79.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighSimilar28761003
Last update: Feb 2026 (version 2.0)