Plant Editosome Database
a curated database of RNA editosome in plants

MEF28 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF28
Synonym: Mitochondrial Editing Factor 28
Description: MEF28 edits two adjacent mitochondrial sites, nad2-89 and nad2-90; When the sDYW domain of MEF28 was replaced with one derived from MEF11 or CRR22, the ability to edit downstream sites was lost, suggesting that the DYW domain of MEF28 provides unique target flexibility for two continuous cytidines; The gating domain of MEF28 plays an important role in specific target site recognition of the DYW domain; This finding suggests that the DYW domain and its internal gating domain fine-tune the specificity of the target site, which is valuable information for designing specific synthetic RNA editing tools based on plant RNA editing factors
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: AT5G06540 (Arabidopsis thaliana)
Edited Gene(s): nad2
Editing Type(s): C-to-U (4)
Publication(s): [1] The Gating Domain of MEF28 Is Essential for Editing Two Contiguous Cytidines in nad2 mRNA in Arabidopsis thaliana., Plant & cell physiology, 2024. [PMID=37530742]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G06540 Mitochondrion nad2 89, 90 CDS C-to-U UCC=>UUU S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbia (Col-0)ColControlControlNo mutantNormalLeafNASequencing RT-PCR products100%, 100%Complete,None, None37530742
SAIL_77_E03mef28-1A T-DNA insertionThe T-DNA insertions of mef28-1 and mef28-2 are located in the DYW domain and the second PPR motif, respectively.HomozygousThe two T-DNA lines for the MEF28 gene do not show visible growth and developmental defects under standard growth conditionsLeafNASequencing RT-PCR products0%, 0%Unedited,Absent, Ab37530742
WiscDsLox461-464F22mef28-2A T-DNA insertionThe T-DNA insertions of mef28-1 and mef28-2 are located in the DYW domain and the second PPR motif, respectively.HomozygousThe two T-DNA lines for the MEF28 gene do not show visible growth and developmental defects under standard growth conditionsLeafNASequencing RT-PCR products0%, 0%Unedited,Absent, Ab37530742
SAIL_77_E03mef28-1+35S:MEF28ComplementationPlants of the mutant line mef28-1 was transformed by dip inoculation with the respective clones of various protein constructs including the chimeras as described previously (Glass et al. 2015). TransgHomozygousNormalLeafNASequencing RT-PCR products100%, 100%Complete,Restored,37530742
Last update: Jul 2021 (version 1.0)