MEF29 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF29
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 29
Description: Encoding a pentatricopeptide repeat protein involved in mitochondrial RNA editing.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G30700 (Arabidopsis thaliana)
Edited Gene(s): nad5    cob
Editing Type(s): C-to-U (12)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] PPR2263, a DYW-Subgroup Pentatricopeptide repeat protein, is required for mitochondrial nad5 and cob transcript editing, mitochondrion biogenesis, and maize growth., The Plant cell, 2012. [PMID=22319053]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G30700 Mitochondrion cob 908 CDS C-to-U CCA/CCA=>CUA/UUA
NA=>NA
P=>L/L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_096438mef29-1T-DNA InsertionThe T-DNA insertion is located in the C-terminal DYW domainHomozygousDevelopment is slightly delayed (e.g., bolting and flower set start ;1 to 2 d later than in wild-type plants)LeafNADirect Sequencing of PCR ProductsNALowDecreased22319053
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Col-0WTControlControlControlNANANARNA-seq98.40%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract98.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract87.00%HighDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT4G30700 Mitochondrion nad5 1550 CDS C-to-U ACC=>AUC
NA=>NA
T=>I
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_096438mef29-1T-DNA InsertionThe T-DNA insertion is located in the C-terminal DYW domainHomozygousDevelopment is slightly delayed (e.g., bolting and flower set start ;1 to 2 d later than in wild-type plants)LeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22319053
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Col-0WTControlControlControlNANANARNA-seq97.57%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)