MEF29 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MEF29
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 29
Description: Encoding a pentatricopeptide repeat protein involved in mitochondrial RNA editing.
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G30700 (Arabidopsis thaliana)
Edited Gene(s): nad5    cob
Editing Type(s): C-to-U (12)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] PPR2263, a DYW-Subgroup Pentatricopeptide repeat protein, is required for mitochondrial nad5 and cob transcript editing, mitochondrion biogenesis, and maize growth., The Plant cell, 2012. [PMID=22319053]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G30700 Mitochondrion cob 908 CDS Arabidopsis Genome Initiative
EnsemblGenomes (release 39)
NA
JF729202.1
BK010421
NA
NA NA C-to-U CCA/CCA=>CUA/UUA
NA=>NA
P=>L/L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_096438mef29-1T-DNA InsertionThe T-DNA insertion is located in the C-terminal DYW domainHomozygousDevelopment is slightly delayed (e.g., bolting and flower set start ;1 to 2 d later than in wild-type plants)LeafNADirect Sequencing of PCR ProductsNALowDecreased22319053
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Col-0WTControlControlControlNANANARNA-seq98.40%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract98.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract87.00%HighDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT4G30700 Mitochondrion nad5 1550 CDS Arabidopsis Genome Initiative
EnsemblGenomes (release 39)
NA
JF729202.1
BK010421
NA
NA NA C-to-U ACC=>AUC
NA=>NA
T=>I
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_096438mef29-1T-DNA InsertionThe T-DNA insertion is located in the C-terminal DYW domainHomozygousDevelopment is slightly delayed (e.g., bolting and flower set start ;1 to 2 d later than in wild-type plants)LeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22319053
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Col-0WTControlControlControlNANANARNA-seq97.57%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Feb 2026 (version 2.0)