Plant Editosome Database
a curated database of RNA editosome in plants

MEF32 - Plant Editosome Database - BIG Data Center
  1. Home
  2. Editing Factors
  3. MEF32

Summary

Editing Factor: MEF32
Synonym: MITOCHONDRIAL EDITING FACTOR 32
Description: Pentatricopeptide repeat (PPR) superfamily protein;(source:Araport11)
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: At4g14170 (Arabidopsis thaliana)
Edited Gene(s): cox2    ccmB    nad1
Editing Type(s): C-to-U (18)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Improved computational target site prediction for pentatricopeptide repeat RNA editing factors, PLoS One, 2013. [PMID=23762347]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At4g14170 Mitochondrion ccmB 569 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANAPCR products were purified by alkaline phosphatase and ExoI100.00%CompleteNone23762347
SALK_039629mef32T-DNA insertionNAKnockoutNANANAPCR products were purified by alkaline phosphatase and ExoI8.00%PoorDecreased23762347
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq93.51%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract27.00%LowNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract1.00%PoorDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract0.00%UneditedAbsent28761003
Arabidopsis thaliana At4g14170 Mitochondrion cox2 27 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANAPCR products were purified by alkaline phosphatase and ExoI78.95%HighNone23762347
SALK_039629mef32T-DNA insertionNAKnockoutNANANAPCR products were purified by alkaline phosphatase and ExoI18.75%PoorDecreased23762347
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq82.62%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract91.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract91.00%HighSimilar28761003
Arabidopsis thaliana At4g14170 Mitochondrion nad1 571 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANAPCR products were purified by alkaline phosphatase and ExoI100.00%CompleteNone23762347
SALK_039629mef32T-DNA insertionNAKnockoutNANANAPCR products were purified by alkaline phosphatase and ExoI0.00%UneditedAbsent23762347
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.59%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)