MEF46 - Plant Editosome Database - BIG Data Center

MEF46 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	MEF46
Synonym:	NA
Description:	Tetratricopeptide repeat (TPR)-like superfamily protein;(source:Araport11)
Protein Family:	PPR
Subclass:	E+
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	At2g17210 (Arabidopsis thaliana)
Edited Gene(s):	nad5
Editing Type(s):	C-to-U (3)
Publication(s):	[1] MEF46 and MEF47 are novel specificity factors for RNA editing sites in mitochondrial nad transcripts, Mitochondrion, 2020. [PMID=32439621]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

At2g17210

Mitochondrion

1958

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	Col-0	No treatment	No treatment	No treatment	Normal	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR. RNA editing levels were e	100.00%	Complete	None	32439621
SALK_059706	mef46-1	A T-DNA Insertion	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG.	Homozygous	The mef46-1 line showed an overall normal growth phenotype comparable to the wild type plant under standard growth chamber conditions.	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR. RNA editing levels were e	0.00%	Unedited	Absent	32439621
SALK_059706	mef46-1+35S:MEF46(1)	Complemented	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG. Overexpression of the MEF46 locus under the 35S promoter in the mef46-1 mutant lin	Homozygous	Normal	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR. RNA editing levels were e	100.00%	Complete	Restored	32439621

Last update: Jul 2021 (version 1.0)