MEF57 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MEF57
Synonym: MITOCHONDRIAL EDITING FACTOR 57
Description: NA
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT5G44230 (Arabidopsis thaliana)
Edited Gene(s): nad9
Editing Type(s): C-to-U (6)
Publication(s): [1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G44230 Mitochondrion nad9 92 CDS NA NA NA NA C-to-U NA=>NA
UCU=>UUU
NA=>NA
S=>F
NA
Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlNo mutantNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract82.00%HighDecreased28761003
Col-0WTControlControlNo mutantNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0mef57A T-DNA InsertionThe T-DNA insertion site is 1,974 bp from the start codon.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract8.11%PoorDecreased28761003
Col-0Complemented mef57ComplementedThe homozygous T-DNA insertion mutant line was transformed with the 35S::MEF57-GFP plasmids (in pK7WGF2 backbone), by the floral-dip method.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteRestored28761003
Last update: Sep 2026 (version 2.0)