MEF57 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MEF57
Synonym: MITOCHONDRIAL EDITING FACTOR 57
Description: Pentatricopeptide repeat (PPR) superfamily protein;(source:Araport11)
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT5G44230 (Arabidopsis thaliana)
Edited Gene(s): nad1    nad9
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G44230 Mitochondrion nad1 743 CDS EnsemblGenomes (release 39) BK010421 NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq89.04%HighNone32098170
Arabidopsis thaliana AT5G44230 Mitochondrion nad9 92 CDS NA NA NA NA C-to-U NA=>NA
UCU=>UUU
NA=>NA
S=>F
NA
Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract82.00%HighDecreased28761003
Col-0WTControlControlControlNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0mef57A T-DNA InsertionThe T-DNA insertion site is 1,974 bp from the start codon.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract8.11%PoorDecreased28761003
Col-0Complemented mef57ComplementedThe homozygous T-DNA insertion mutant line was transformed with the 35S::MEF57-GFP plasmids (in pK7WGF2 backbone), by the floral-dip method.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteRestored28761003
Last update: Feb 2026 (version 2.0)