MEF57 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF57
Synonym: NA
Description: Pentatricopeptide repeat (PPR) superfamily protein;(source:Araport11)
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: At5g44230 (Arabidopsis thaliana)
Edited Gene(s): nad1    nad9
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At5g44230 Mitochondrion nad1 743 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq89.04%HighNone32098170
Arabidopsis thaliana At5g44230 Mitochondrion nad9 92 CDS C-to-U NA=>NA
UCU=>UUU
NA=>NA
S=>F
NA
Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract88.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract82.00%HighDecreased28761003
Col-0WTNo treatmentNo treatmentNo treatmentNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0mef57A T-DNA InsertionThe T-DNA insertion site is 1,974 bp from the start codon.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract8.11%PoorDecreased28761003
Col-0Complemented mef57ComplementedThe homozygous T-DNA insertion mutant line was transformed with the 35S::MEF57-GFP plasmids (in pK7WGF2 backbone), by the floral-dip method.HomozygousNANANART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteRestored28761003
Last update: Jul 2021 (version 1.0)