Plant Editosome Database
a curated database of RNA editosome in plants

MEF7 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF7
Synonym: MITOCHONDRIAL EDITING FACTOR 7
Description: Encodes a DYW-class PPR protein required for RNA editing at four sites in mitochondria of Arabidopsis thaliana.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: AT5G09950 (Arabidopsis thaliana)
Edited Gene(s): ccmB    cob    nad2    nad4L    nad4
Editing Type(s): C-to-U (33)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The DYW-class PPR protein MEF7 is required for RNA editing at four sites in mitochondria of Arabidopsis thaliana., RNA Biology, 2012. [PMID=22258224]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G09950 Mitochondrion ccmB 28 CDS C-to-U CAU=>UAU
NA=>NA		 H=>Y
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22258224
Colmef7-1EMS MutantThe EMS mutant causes single amino acid exchange (G->R) in the PLS repeat regionHomozygousGrow normallyLeafNADirect Sequencing of PCR ProductsNAPoorDecreased22258224
Colmef7-2EMS MutantThe EMS mutant causes single amino acid exchange (W->Stop) in the PLS repeat regionNAGrow normallyLeafNADirect Sequencing of PCR ProductsNAPoorDecreased22258224
SALK_041847mef7-3T-DNA InsertionT-DNA is inserted in the PLS repeat regionHomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq90.55%HighNone32098170
Arabidopsis thaliana AT5G09950 Mitochondrion cob 325 CDS C-to-U CAU=>UAU
NA=>NA		 H=>Y
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22258224
Colmef7-1EMS MutantThe EMS mutant causes single amino acid exchange (G->R) in the PLS repeat regionHomozygousGrow normallyLeafNADirect Sequencing of PCR ProductsNAPoorDecreased22258224
Colmef7-2EMS MutantThe EMS mutant causes single amino acid exchange (W->Stop) in the PLS repeat regionNAGrow normallyLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
SALK_041847mef7-3T-DNA InsertionT-DNA is inserted in the PLS repeat regionHomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.07%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT5G09950 Mitochondrion nad2 1433 CDS C-to-U UCA=>UUA
NA=>NA		 S=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22258224
Colmef7-1EMS MutantThe EMS mutant causes single amino acid exchange (G->R) in the PLS repeat regionHomozygousGrow normallyLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
Colmef7-2EMS MutantThe EMS mutant causes single amino acid exchange (W->Stop) in the PLS repeat regionNAGrow normallyLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
SALK_041847mef7-3T-DNA InsertionT-DNA is inserted in the PLS repeat regionHomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq97.00%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT5G09950 Mitochondrion nad4 896 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.58%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighSimilar28761003
Arabidopsis thaliana AT5G09950 Mitochondrion nad4L 41 CDS C-to-U UCU=>UUU
NA=>NA		 S=>F
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22258224
Colmef7-1EMS MutantThe EMS mutant causes single amino acid exchange (G->R) in the PLS repeat regionHomozygousGrow normallyLeafNADirect Sequencing of PCR ProductsNALowDecreased22258224
Colmef7-2EMS MutantThe EMS mutant causes single amino acid exchange (W->Stop) in the PLS repeat regionNAGrow normallyLeafNADirect Sequencing of PCR ProductsNALowDecreased22258224
SALK_041847mef7-3T-DNA InsertionT-DNA is inserted in the PLS repeat regionHomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22258224
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.53%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)