MEF8S - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF8S
Synonym: MEF8 SIMILAR
Description: Encoding a protein involved in RNA editing at two specific sites in plant mitochondria. Mutants of MEF8S show reduced RNA editing in pollen but not in leaves.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G32450 (Arabidopsis thaliana)
Edited Gene(s): nad5    nad6
Editing Type(s): C-to-U (10)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Two related RNA-editing proteins target the same sites in mitochondria of Arabidopsis thaliana., The Journal of biological chemistry, 2012. [PMID=22977245]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G32450 Mitochondrion nad5 676 CDS C-to-U CUU=>UUU
NA=>NA
L=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22977245
ColWTControlControlControlNormalPollenNADirect Sequencing of PCR Products100.00%CompleteNone22977245
SALK_047005Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNALeafNADirect Sequencing of PCR Products100.00%CompleteSimilar22977245
SALK_047005Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNAPollenNADirect Sequencing of PCR Products25.00%LowDecreased22977245
Col-0WTControlControlControlNANANARNA-seq99.38%HighNone32098170
Arabidopsis thaliana AT4G32450 Mitochondrion nad6 95 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNone22977245
ColWTControlControlControlNormalPollenNADirect Sequencing of PCR Products100.00%CompleteNone22977245
SALK_047006Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNALeafNADirect Sequencing of PCR Products100.00%CompleteSimilar22977245
SALK_047006Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNAPollenNADirect Sequencing of PCR Products40.00%MediumDecreased22977245
Col-0WTControlControlControlNANANARNA-seq99.01%HighNone32098170
Last update: Jul 2021 (version 1.0)