MEF8S - Plant Editosome Database

Editing Factor:	MEF8S
Synonym:	MEF8 SIMILAR
Description:	Mutants of MEF8 show diminished editing in leaves but not in pollen, whereas mutants of the related protein MEF8S show reduced RNA editing in pollen but not in leaves; Overexpressed MEF8 or MEF8S both increase editing at the two target sites in a mef8 mutant; Double mutants of MEF8 and MEF8S are not viable although both identified target sites are in mRNAs for nonessential proteins
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT4G32450 (Arabidopsis thaliana)
Edited Gene(s):	nad5 nad6
Editing Type(s):	C-to-U (8)
Publication(s):	[1] Two Related RNA-editing Proteins Target the Same Sites in Mitochondria of Arabidopsis thaliana, The Journal of Biological Chemistry, 2012. [doi:10.1074/jbc.M112.397992]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G32450

Mitochondrion

676

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
Col	WT	Control	Control	No mutant	Normal	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
SALK_047005C	mef8s-1	T-DNA Insertion	T-DNA is inserted in the open reading frame coding for the N-terminal region	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	22977245
SALK_047005C	mef8s-1	T-DNA Insertion	T-DNA is inserted in the open reading frame coding for the N-terminal region	Homozygous	NA	Pollen	NA	Direct Sequencing of PCR Products	25.00%	Low	Decreased	22977245

Arabidopsis thaliana

AT4G32450

Mitochondrion

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
Col	WT	Control	Control	No mutant	Normal	Pollen	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	22977245
SALK_047006C	mef8s-1	T-DNA Insertion	T-DNA is inserted in the open reading frame coding for the N-terminal region	Homozygous	NA	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	Similar	22977245
SALK_047006C	mef8s-1	T-DNA Insertion	T-DNA is inserted in the open reading frame coding for the N-terminal region	Homozygous	NA	Pollen	NA	Direct Sequencing of PCR Products	40.00%	Medium	Decreased	22977245