MEF8S - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF8S
Synonym: MEF8 SIMILAR
Description: Encodes a protein involved in RNA editing at two specific sites in plant mitochondria. Mutants of MEF8S show reduced RNA editing in pollen but not in leaves.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G32450 (Arabidopsis thaliana)
Edited Gene(s): nad5    nad6
Editing Type(s): C-to-U (10)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Two Related RNA-editing Proteins Target the Same Sites in Mitochondria of Arabidopsis thaliana, The Journal of Biological Chemistry, 2012. [PMID=22977245]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G32450 Mitochondrion nad5 676 CDS C-to-U CUU=>UUU
NA=>NA
L=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22977245
ColWTNo treatmentNo treatmentNo treatmentNo treatmentPollenNADirect Sequencing of PCR Products100.00%CompleteNone22977245
SALK_047005Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNALeafNADirect Sequencing of PCR Products100.00%CompleteSimilar22977245
SALK_047005Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNAPollenNADirect Sequencing of PCR Products25.00%LowDecreased22977245
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.38%HighNone32098170
Arabidopsis thaliana AT4G32450 Mitochondrion nad6 95 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22977245
ColWTNo treatmentNo treatmentNo treatmentNo treatmentPollenNADirect Sequencing of PCR Products100.00%CompleteNone22977245
SALK_047006Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNALeafNADirect Sequencing of PCR Products100.00%CompleteSimilar22977245
SALK_047006Cmef8s-1T-DNA InsertionT-DNA is inserted in the open reading frame coding for the N-terminal regionHomozygousNAPollenNADirect Sequencing of PCR Products40.00%MediumDecreased22977245
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.01%HighNone32098170
Last update: Jul 2021 (version 1.0)