MREF1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MREF1
Synonym: NA
Description: Tetratricopeptide repeat (TPR)-like superfamily protein;(source:Araport11)
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: At4g04370 (Arabidopsis thaliana)
Edited Gene(s): nad5
Editing Type(s): C-to-U (3)
Publication(s): [1] Identification of new pentatricopeptide repeat proteins, MREF1 and 2, involved in mitochondrial RNA editing, using computational target RNA prediction, Plant Biotechnol (Tokyo), 2020. [PMID=32362757]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At4g04370 Mitochondrion nad5 242 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColumbiaWTNo treatmentNo treatmentNo treatmentNASeedling2 weeksSanger sequencing100.00%CompleteNone32362757
SALK_025427CSALK_025427CT-DNA insertionThe T-DNA insertion was located in coding regionsHomozygousTwo-week-old seedlings of the gene-deficient lines displayed no visible aberrant phenotypes under these experimental conditions.Seedling2 weeksSanger sequencing0.00%UneditedAbsent32362757
SALK_025427CComplementedComplementationPPR gene-deficient lines were transformed using the floral dip method, with a construct that expresses the corresponding wild-type PPR protein under the control of the A. thaliana ubiquitin-10 promoteHomozygousNASeedling2 weeksSanger sequencing100.00%CompleteRestored32362757
Last update: Jul 2021 (version 1.0)